A search for c-myb gene regulatory sequences: cloning and restriction analysis of the 18-kb BamHI fragment of chicken chromosomal DNA, containing the 5' part of the c-myb gene
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
2731613
Knihovny.cz E-resources
- MeSH
- Chromosomes MeSH
- Deoxyribonuclease BamHI MeSH
- DNA genetics MeSH
- Cloning, Molecular MeSH
- Chickens MeSH
- Proto-Oncogenes * MeSH
- Regulatory Sequences, Nucleic Acid * MeSH
- Restriction Mapping MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Deoxyribonuclease BamHI MeSH
- DNA MeSH
The 18-kb BamHI fragment of the chicken chromosomal DNA derived from the 5' end of the myb proto-oncogene has been cloned. The 5' part of this clone represented by a 7.2-kb BamHI-EcoRI fragment has been analysed by means of restriction mapping, which revealed the existence of about 2.5 kb of the CpG dinucleotide-rich sequence within this fragment. A short probe prepared from the CpG-rich sequence hybridizes with the 3.6-kb c-myb mRNA. Based on our results and published c-myb cDNA sequence, we conclude that the cloned fragment contains c-myb promoter.
Transcription of the chicken myb proto-oncogene starts within a CpG island
