A search for c-myb gene regulatory sequences: cloning and restriction analysis of the 18-kb BamHI fragment of chicken chromosomal DNA, containing the 5' part of the c-myb gene
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
2731613
Knihovny.cz E-zdroje
- MeSH
- chromozomy MeSH
- deoxyribonukleasa BamHI MeSH
- DNA genetika MeSH
- klonování DNA MeSH
- kur domácí MeSH
- protoonkogeny * MeSH
- regulační oblasti nukleových kyselin * MeSH
- restrikční mapování MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- deoxyribonukleasa BamHI MeSH
- DNA MeSH
The 18-kb BamHI fragment of the chicken chromosomal DNA derived from the 5' end of the myb proto-oncogene has been cloned. The 5' part of this clone represented by a 7.2-kb BamHI-EcoRI fragment has been analysed by means of restriction mapping, which revealed the existence of about 2.5 kb of the CpG dinucleotide-rich sequence within this fragment. A short probe prepared from the CpG-rich sequence hybridizes with the 3.6-kb c-myb mRNA. Based on our results and published c-myb cDNA sequence, we conclude that the cloned fragment contains c-myb promoter.
Transcription of the chicken myb proto-oncogene starts within a CpG island
