Demonstration of proteinase inhibitors cystatin A, B and C in breast cancer and in cell lines MCF-7 and ZR-75-1
Language English Country Slovakia Media print
Document type Journal Article
PubMed
2785657
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Cystatin B MeSH
- Cystatin C MeSH
- Cystatins * MeSH
- Estradiol pharmacology MeSH
- Cysteine Proteinase Inhibitors * MeSH
- Protease Inhibitors analysis biosynthesis MeSH
- Humans MeSH
- Tumor Cells, Cultured analysis MeSH
- Breast Neoplasms analysis metabolism MeSH
- Proteins analysis MeSH
- Protein Biosynthesis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- CST3 protein, human MeSH Browser
- CSTB protein, human MeSH Browser
- Cystatin B MeSH
- Cystatin C MeSH
- Cystatins * MeSH
- Estradiol MeSH
- Cysteine Proteinase Inhibitors * MeSH
- Protease Inhibitors MeSH
- Proteins MeSH
Polyclonal rabbit antibodies to endogenous natural inhibitors of cysteine proteinases (cystatins) were used for their immunohistochemical demonstration in samples of breast cancers and in two breast cancer derived cell lines (MCF-7 and ZR-75-1). The influence of estrogen stimulation on the expression of these inhibitors was also studied. The results showed that all cystatins tested can be found equally inside carcinomatous cells or in surrounding connective tissue, as well as in both cell lines examined. Further, the expression of inhibitors can be regulated by estrogen, and a certain role of cystatins in the regulation of the mitotic activity and differentiation of mammary cells can be supposed.
