Electrophoretic study of alpha-D-galactosidases from seeds of Glycine soja and Vigna radiata possessing erythroagglutinating activity
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
- MeSH
- alfa-galaktosidasa analýza MeSH
- chromatografie afinitní MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- galaktosidasy analýza MeSH
- Glycine max enzymologie MeSH
- koncentrace vodíkových iontů MeSH
- lektiny analýza MeSH
- molekulová hmotnost MeSH
- rostlinné lektiny MeSH
- semena rostlinná enzymologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- alfa-galaktosidasa MeSH
- galaktosidasy MeSH
- lektiny MeSH
- rostlinné lektiny MeSH
Polyacrylamide gel electrophoresis in an acidic buffer system was used to study the electrophoretic behaviour of two forms of alpha-D-galactosidase from seeds of soy bean (Glycine soja) and mung bean (Vigna radiata). The interaction of the enzymes with saccharides was monitored by affinity electrophoresis; for the preparation of affinity gels, water-soluble O-glycosyl polyacrylamide copolymers and polysaccharides were used. alpha-D-Galactosidases from both sources interact with immobilized alpha-D-galactosyl residues. On the basis of the results of affinity electrophoresis performed in the presence of various free sugars, dissociation constants for the complexes between alpha-D-galactosidase and free sugars were calculated.
Citace poskytuje Crossref.org
