Avian nephroblastomas induced by a retrovirus (MAV-2) lacking oncogene. I. Construction of MAV-1 and MAV-2 proviral restriction maps and preparation of specific proviral molecular subclones
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
2849567
Knihovny.cz E-zdroje
- MeSH
- DNA nádorová genetika MeSH
- DNA virů genetika MeSH
- klonování DNA * MeSH
- kur domácí MeSH
- nádory ledvin mikrobiologie MeSH
- onkogeny MeSH
- pomocné viry genetika MeSH
- proviry genetika MeSH
- restrikční enzymy MeSH
- virové geny * MeSH
- virus ptačí leukózy genetika MeSH
- virus ptačí myeloblastózy genetika MeSH
- Wilmsův nádor mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA nádorová MeSH
- DNA virů MeSH
- restrikční enzymy MeSH
A 9.8 kb DNA fragment containing the complete MAV-1 provirus was recloned from the recombinant bacteriophage lambda 311411 (Perbal et al., 1985) into the plasmid pAT153. A detailed and precise restriction map of the obtained clone (pAT-MAV-1) was constructed. From compilation of this map and the known sequence of a variable portion of the MAV-2 env gene was a restriction map of MAV-2 deduced. Knowledge of the detailed pAT-MAV-1 map facilitated the preparation of five specific proviral subclones: pAT-U3 and pUC-U3 (both contain the U3 domain of the proviral LTR, which is MAV-specific and displays no homology with other hitherto known retroviruses including avian endogenous proviruses), pUC-RU5 (containing the R and U5 domains of the proviral LTR), pUC-UT5 (containing untranslated sequences flanking the 5' LTR), and pUC-UT3 (containing untranslated sequences flanking the 3' LTR). Thus tools for analysis of integrated MAV-2 proviruses in nephroblastomas induced by this virus were formed.
