The effect of phospholipase A2 and of related agents on ouabain binding and Na, K-ATPase activity were studied in intact and detergent-treated membrane preparations of rat brain cortex and pig kidney medulla. It was found that phospholipase A2 (PLA2) may distinguish or dissociate ouabain binding complexes I (ATP + Mg + Na) and II (Pi + Mg), stimulating the former and inhibiting the latter. Procedures which break the permeability barriers of vesicular membrane preparations, such as repeated freezing-thawing, sonication or hypoosmotic shock failed to mimic the effect of PLA2, indicating that it was not acting primarily by opening the inside-out oriented vesicles. The detergent digitonin exhibited similar effects on ouabain binding in both ATP + Mg + Na and Pi + Mg media. Other detergents were ineffective. The ability of PLA2 to distinguish between ouabain binding type I and II can be manifested even in SDS-treated, purified preparations of Na, K-ATPase. The number of ATP + Mg + Na-dependent sites is unchanged, while the Pi + Mg-dependent sites are decreased in number in a manner similar to that seen in original membranes. This inhibition is completely lost in the reconstituted Na, K-ATPase system, where the ATP- as well as Pi-oriented ouabain sites are inhibited by PLA2.

Institute of Physiology Czechoslovak Academy of Sciences Praha

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