Protein turnover and proteolysis during sporulation of Bacillus subtilis
Language English Country United States Media print
Document type Journal Article, Research Support, U.S. Gov't, P.H.S.
Grant support
1-506-RR-08316
NCRR NIH HHS - United States
5R01 19643
PHS HHS - United States
PubMed
3125094
DOI
10.1007/bf02877199
Knihovny.cz E-resources
- MeSH
- Bacillus subtilis enzymology metabolism physiology MeSH
- Bacterial Proteins analysis biosynthesis metabolism MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Endopeptidases metabolism MeSH
- Isoelectric Focusing MeSH
- Hydrogen-Ion Concentration MeSH
- Leucine analysis metabolism MeSH
- Mathematics MeSH
- Molecular Weight MeSH
- Spores, Bacterial MeSH
- Publication type
- Journal Article MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Endopeptidases MeSH
- Leucine MeSH
A two-dimensional electrophoretic method was used to show that protein degradation occurs immediately after the end of exponential growth but that its occurrence is masked in the usual assay methods for a 2-h period and that degradation is apparently nonselective with respect to protein molar mass or charge. The results suggest that considerable reutilization of internal amino acids may occur during sporulation regardless of the size of the external chase. Finally, the levels of intracellular proteinase activities present even at the end of exponential phase growth, as measured in vitro, are sufficient to account for the maximum rates of protein degradation observed in vivo.
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