1-Methyldodecyldimethylamine oxide (MDDO) and N,N'-bis(dodecyldimethyl)-1,2-ethanediammonium dibromide (BDED) exhibit a significant affinity for the surface of Bacillus cereus spores and adsorb very rapidly to the cells; they have a pronounced inhibitory effect on spore outgrowth. In order to alter the affinity of the spore surface for these inhibitors, the spores were pretreated with sodium dodecyl sulfate (SDS), and with an electronegative (Tween 80) and electropositive (histone) compound. In SDS-pretreated spores the inhibitory effect of MDDO and BDED was abolished to a considerable extent. Whereas the development of intact spores was inhibited already after germination, in SDS-pretreated spores the postgermination development continued but was not completed. In Tween 80-pretreated spores the addition of BDED led only to a retardation of outgrowth and division; BDED added only during the division stage interrupted further development completely. Histone-pretreated spores stopped their development instantaneously after the addition of BDED at any phase of the postgermination development. The possible mechanisms of the interaction of the compounds used with spore surface or rather with the state of its structures are discussed.

Department of Biochemistry and Microbiology Pharmaceutical Faculty Comenius University Bratislava Czechoslovakia

Zobrazit více v PubMed

Folia Microbiol (Praha). 1966;11(4):314-22 PubMed

Folia Microbiol (Praha). 1981;26(3):189-95 PubMed

Folia Microbiol (Praha). 1967;12(3):301-7 PubMed

Pharm Acta Helv. 1971 Oct-Nov;46(10):671-6 PubMed

Microbios. 1981;30(119):27-35 PubMed

Biosystems. 1975 Mar;6(3):176-87 PubMed

Adaptive changes in fatty acids of E. coli strains exposed to a quaternary ammonium salt and an amine oxide

Activation of human leukocytes by lipid A from E. coli strains adapted to quaternary ammonium salt and amine oxide

Adsorption on bacterial spores depending on the aggregation properties of antimicrobial tensides