Induction and regulation of cytotoxic T cells by microbial antigens and recombinant interleukin 2
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
3149595
Knihovny.cz E-zdroje
- MeSH
- antigeny bakteriální farmakologie MeSH
- buněčné dělení účinky léků MeSH
- Candida albicans MeSH
- cytotoxické T-lymfocyty cytologie účinky léků imunologie MeSH
- dospělí MeSH
- HLA antigeny metabolismus farmakologie MeSH
- interleukin-2 farmakologie MeSH
- lidé MeSH
- monoklonální protilátky MeSH
- Mycobacterium tuberculosis MeSH
- rekombinantní proteiny farmakologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny bakteriální MeSH
- HLA antigeny MeSH
- interleukin-2 MeSH
- monoklonální protilátky MeSH
- rekombinantní proteiny MeSH
The proliferation and development of cytotoxic T cells was investigated in human peripheral blood mononuclear cell (PBMC) cultures stimulated with an antigenic extract from Candida albicans (MPPS), or with the purified protein derivative from Mycobacterium tuberculosis (PPD), or with human recombinant interleukin 2 (rIL-2). Microbial antigen- and rIL-2-induced cytotoxic T cells were able to lyse both natural killer (NK) sensitive and resistant targets. No correlation was observed between the development of T cell cytotoxicity and interferon (IFN) production in vitro. The addition of anti-class II monoclonal antibodies at the beginning of MPPS/PPD-stimulated cultures inhibited the cell proliferation, IFN production and T cell cytotoxicity, while all these cellular activities were not inhibited by anti-class II antibodies in rIL-2-stimulated cultures. Finally, antibodies to class I determinants inhibit T cell cytotoxicity, suggesting a role of such determinants in the development of the non-adaptive immunity to microbial infections.