Gas chromatographic method for the determination of N-acetyl-S-(N-methylcarbamoyl)cysteine, a metabolite of N,N-dimethylformamide and N-methylformamide, in human urine
Language English Country Netherlands Media print
Document type Journal Article
- MeSH
- Acetylcysteine analogs & derivatives urine MeSH
- Chromatography, Gas MeSH
- Dimethylformamide urine MeSH
- Formamides urine MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acetylcysteine MeSH
- Dimethylformamide MeSH
- Formamides MeSH
- methylformamide MeSH Browser
- N-acetyl-S-(N-methylcarbamoyl)cysteine MeSH Browser
A simple method has been developed for the determination of N-acetyl-S-(N-methylcarbamoyl)cysteine in human urine. Treatment of a urine sample (1 ml) with ethanol (2 ml) and potassium carbonate (1.5 g) produces ethyl N-methylcarbamate, which is extracted into ethanol and measured by packed column gas chromatography with nitrogen-sensitive detection. The limit of quantitation in human urine is 1 microgram/ml and the between-sample coefficient of variation is 5-11%. Simultaneously, N,N-dimethylformamide, N-methylformamide and formamide can also be determined.
References provided by Crossref.org
Absorption, metabolism and elimination of N,N-dimethylformamide in humans
Percutaneous absorption of N,N-dimethylformamide in humans
