Gas chromatographic method for the determination of N-acetyl-S-(N-methylcarbamoyl)cysteine, a metabolite of N,N-dimethylformamide and N-methylformamide, in human urine
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články
- MeSH
- acetylcystein analogy a deriváty moč MeSH
- chromatografie plynová MeSH
- dimethylformamid moč MeSH
- formamidy moč MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- acetylcystein MeSH
- dimethylformamid MeSH
- formamidy MeSH
- methylformamide MeSH Prohlížeč
- N-acetyl-S-(N-methylcarbamoyl)cysteine MeSH Prohlížeč
A simple method has been developed for the determination of N-acetyl-S-(N-methylcarbamoyl)cysteine in human urine. Treatment of a urine sample (1 ml) with ethanol (2 ml) and potassium carbonate (1.5 g) produces ethyl N-methylcarbamate, which is extracted into ethanol and measured by packed column gas chromatography with nitrogen-sensitive detection. The limit of quantitation in human urine is 1 microgram/ml and the between-sample coefficient of variation is 5-11%. Simultaneously, N,N-dimethylformamide, N-methylformamide and formamide can also be determined.
Citace poskytuje Crossref.org
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