A rapid method for isolation of double stranded RNA
Preparative biochemistry.
1987 ;
17 (4) :
423-33.
Language English Country United States Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid03501861
- MeSH
- RNA, Double-Stranded isolation & purification MeSH
- Phenol MeSH
- Phenols MeSH
- Hydrogen-Ion Concentration MeSH
- Trichomonas vaginalis analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- RNA, Double-Stranded MeSH
- Phenol MeSH
- Phenols MeSH
A rapid and simple method for the isolation and purification of dsRNA is presented. The crucial step of this method is the extraction of proteins and DNA with acid phenol. After the extraction, only RNA is left in the aqueous phase. ssRNA contamination of the RNA preparation can be greatly reduced when ammonium sulfate is present during the extraction.
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