The existence of six dsRNA segments of Trichomonas vaginalis virus was confirmed and the molar mass and relative abundance of these segments were determined by agarose gel electrophoresis with reovirus dsRNA serving as a standard. The M's were 3.5, 3.4, 3.2, 2.5, 1.4 and 0.34 Mg/mol for the two strains studied, the relative abundances, however, were 1.0, 1.4, 3.0, 0.3, 2.7, 4.2 and 1.0, 0.6, 1.7, 0.5, 3.4 1.0 for these strains, respectively. Cell homogenate fractionation showed that all dsRNA segments were associated with viral particles. The data appeared to support the hypothesis of a relationship between viruses of the protozoan T. vaginalis and of the yeast Saccharomyces cerevisiae.

Institute of Molecular Genetics Czechoslovak Academy of Sciences Prague

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Infect Immun. 1986 Aug;53(2):285-93 PubMed

Biopolymers. 1984 Dec;23(12):2725-42 PubMed

Z Naturforsch C. 1978 Jan-Feb;33(1-2):124-38 PubMed

Prep Biochem. 1987;17(4):423-33 PubMed

J Parasitol. 1957 Aug;43(4):488-90 PubMed

Proc Natl Acad Sci U S A. 1986 Oct;83(20):7956-60 PubMed

Folia Microbiol (Praha). 1987;32(4):345-8 PubMed

J Exp Med. 1987 Jul 1;166(1):142-50 PubMed

Antimicrob Agents Chemother. 1979 Feb;15(2):254-7 PubMed

J Biol Chem. 1985 Mar 25;260(6):3697-702 PubMed

Infect Immun. 1985 Dec;50(3):701-8 PubMed

Microbiol Rev. 1984 Jun;48(2):125-56 PubMed