A radiometric assay for phenylethanolamine N-methyltransferase and catechol O-methyltransferase in a single tissue sample: application to rat hypothalamic nuclei, pineal gland, and heart
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
3674382
DOI
10.1016/0003-2697(87)90503-3
PII: 0003-2697(87)90503-3
Knihovny.cz E-zdroje
- MeSH
- epifýza mozková enzymologie MeSH
- fenylethanolamin-N-methyltransferasa analýza MeSH
- hypothalamus enzymologie MeSH
- inbrední kmeny potkanů MeSH
- katechol-O-methyltransferasa analýza MeSH
- krysa rodu Rattus MeSH
- myokard enzymologie MeSH
- radiometrie metody MeSH
- S-adenosylmethionin MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fenylethanolamin-N-methyltransferasa MeSH
- katechol-O-methyltransferasa MeSH
- S-adenosylmethionin MeSH
A simple and highly sensitive method for simultaneous assay of phenylethanolamine N-methyltransferase (PNMT) and catechol O-methyltransferase (COMT) is described. These enzymes are determined in a single tissue homogenate using S-[methyl-3H] adenosyl-L-methionine as methyl donor and sequentially incubating with the substrates phenylethanolamine and epinephrine. The radioactive products of the enzymatic reactions, N-methylphenylethanolamine and metanephrine, are extracted and then separated by thin-layer chromatography. The identity of the reaction products has been established chromatographically and the conditions for both enzymatic reactions in the assay procedure have been defined. Measurement of PNMT activity in the rat pineal gland or in minute fragments of other tissues (e.g., brain nuclei) has not been possible using previously described methods. Activities of PNMT and COMT in the rat pineal gland, various hypothalamic nuclei, and the auricular and ventricular myocardia are herein reported.
Citace poskytuje Crossref.org
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