Catecholaminergic neurons in the rat intrinsic cardiac nervous system
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12675149
DOI
10.1023/a:1022837810357
Knihovny.cz E-zdroje
- MeSH
- dopamin-beta-hydroxylasa genetika metabolismus MeSH
- fenylethanolamin-N-methyltransferasa genetika metabolismus MeSH
- imunohistochemie MeSH
- katecholaminy metabolismus MeSH
- krysa rodu Rattus MeSH
- messenger RNA metabolismus MeSH
- nervový systém - fyziologické jevy MeSH
- neurony fyziologie MeSH
- neuropeptid Y metabolismus MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- potkani Wistar MeSH
- srdce inervace MeSH
- tyrosin-3-monooxygenasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dopamin-beta-hydroxylasa MeSH
- fenylethanolamin-N-methyltransferasa MeSH
- katecholaminy MeSH
- messenger RNA MeSH
- neuropeptid Y MeSH
- tyrosin-3-monooxygenasa MeSH
Immunoreactivities (IR) for catecholamine-synthesizing enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DbetaH), phenylethanolamine N-methyl transferase (PNMT), serotonin-synthesizing enzyme tryptophan hydroxylase, and neuropeptide Y were investigated in the intrinsic cardiac nervous system of 27-40-day-old rats using fluorescent immunohistochemistry. Individual neurons were identified by the general neuronal marker protein gene product 9.5. The presence of DbetaH and PNMT in the atrial specimens was verified using reverse transcriptase-polymerase chain reaction. Two types of catecholamine-handling intrinsic ganglion neurons were observed: small intensely fluorescent (SIF) cells and large-diameter neurons. SIF cells exhibited TH- and tryptophan hydroxylase-IR, but they were not positive for DbetaH. In contrast, large-diameter intrinsic TH-positive neurons, showing in majority also NPY-IR, displayed also DbetaH- and PNMT-IR, thus indicating the capacity for the synthesis of norepinephrine and epinephrine, respectively. In conclusion, the SIF cells are most probably dopaminergic and serotonergic neurons, whereas large-diameter intrinsic cells seem to represent a subpopulation of norepinephrine- and/or epinephrine-secreting neurons.
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