Hybridoma cultivation in defined serum-free media: growth-supporting substances. I. Transferrin
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
4018305
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení účinky léků MeSH
- hybridomy účinky léků MeSH
- kultivační média farmakologie MeSH
- kultivační techniky metody MeSH
- myši MeSH
- transferin farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kultivační média MeSH
- transferin MeSH
Effect of transferrin and effect of transferrin omission on growth of hybridomas in serum-free medium were studied. Transferrin at concentration 0.5 microgram/ml and higher concentrations stimulated growth of hybridoma cells. The optimum growth-supporting concentration of transferrin was 5 micrograms/ml. Omission of transferrin led to death of hybridoma T3-03 cells within 3-4 days. Omission of transferrin for a period of only 24 h damaged cells so severely, that they were unable to recover fully.
Growth of human tumor cell lines in transferrin-free, low-iron medium
