Hybridoma cultivation in defined serum-free media: growth-supporting substances. I. Transferrin
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
4018305
Knihovny.cz E-resources
- MeSH
- Cell Division drug effects MeSH
- Hybridomas drug effects MeSH
- Culture Media pharmacology MeSH
- Culture Techniques methods MeSH
- Mice MeSH
- Transferrin pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Culture Media MeSH
- Transferrin MeSH
Effect of transferrin and effect of transferrin omission on growth of hybridomas in serum-free medium were studied. Transferrin at concentration 0.5 microgram/ml and higher concentrations stimulated growth of hybridoma cells. The optimum growth-supporting concentration of transferrin was 5 micrograms/ml. Omission of transferrin led to death of hybridoma T3-03 cells within 3-4 days. Omission of transferrin for a period of only 24 h damaged cells so severely, that they were unable to recover fully.
Growth of human tumor cell lines in transferrin-free, low-iron medium
