Heavy chain disease proteins (FOR) were isolated from human plasma. These proteins were also detected immunochemically in the urine of the patient. The proteins were disulphide-linked Fc-like dimers with molar mass 64.2 kg/mol and sedimentation rate S020,W = 0.356 ps (3.56 S). Similar amounts of aspartic acid and pyroglutamic acid were found at the N-terminus. After cyanogen bromide cleavage of the FOR proteins, three peptides were isolated and their amino acid composition and partial amino acid sequence was determined. We suggest that two Fc-like proteins of similar sizes are present in the plasma: (1) the first with N-terminal aspartic acid corresponding to position 221 of gamma 1 EU chain and (2) the second with N-terminal pyroglutamic acid. The first protein and small amounts of related low-molar mass fragments found also in the plasma could be degradation products of the second protein. Evidence is given on structural differences between the FOR proteins and the corresponding portion of the gamma 1 EU chain.

Zobrazit více v PubMed

Proc Natl Acad Sci U S A. 1979 Jan;76(1):452-6 PubMed

Biochem J. 1951 Jan;48(1):126-8 PubMed

Hoppe Seylers Z Physiol Chem. 1976 Nov;357(11):1571-604 PubMed

J Exp Med. 1939 Jan 1;69(1):103-18 PubMed

Nature. 1979 Feb 22;277(5698):627-33 PubMed

Biochemistry. 1973 Aug 14;12(17):3355-9 PubMed

Biochem J. 1966 Aug;100(2):303-8 PubMed

Scand J Immunol. 1980;11(6):601-7 PubMed

Proc Natl Acad Sci U S A. 1969 May;63(1):78-85 PubMed

Proc Natl Acad Sci U S A. 1971 Jan;68(1):187-91 PubMed

Biochemistry. 1968 Oct;7(10):3340-4 PubMed

J Biol Chem. 1948 Oct;176(1):367-88 PubMed

Biochim Biophys Acta. 1967 Feb 21;133(2):369-70 PubMed

Am J Med. 1964 Sep;37:332-50 PubMed

J Biol Chem. 1969 Aug 25;244(16):4406-12 PubMed

Ann N Y Acad Sci. 1971 Dec 31;190:467-71 PubMed

Biochemistry. 1964 Mar;3:297-317 PubMed

Nature. 1969 Mar 22;221(5186):1166-9 PubMed

Cesk Epidemiol Mikrobiol Imunol. 1958 Nov;7(6):414-7 PubMed

Nature. 1978 Jun 1;273(5661):400-1 PubMed

Scand J Immunol. 1972;1(1):53-62 PubMed

Immunochemistry. 1976 Mar;13(3):245-9 PubMed

Blood. 1970 Aug;36(2):137-44 PubMed

Immunol Today. 1980 Jul;1(1):10-7 PubMed

Immunochemistry. 1969 Jan;6(1):53-66 PubMed

J Immunol Methods. 1972 Nov;2(1):67-74 PubMed

Nature. 1978 Dec 21-28;276(5690):790-5 PubMed

Science. 1968 Dec 20;162(3860):1396-7 PubMed

Biochemistry. 1968 May;7(5):1959-66 PubMed

J Immunol. 1978 Dec;121(6):2582-3 PubMed

Science. 1966 Feb 25;151(3713):988-90 PubMed

Arch Biochem Biophys. 1969 Nov;134(2):279-84 PubMed

Biochem J. 1945;39(5):507-15 PubMed

FEBS Lett. 1972 Feb 15;20(3):321-323 PubMed