Preparation of zymosan from yeast cell walls
Language English Country United States Media print
Document type Journal Article
PubMed
7002749
DOI
10.1007/bf02897218
Knihovny.cz E-resources
- MeSH
- Cell Wall analysis MeSH
- Methods MeSH
- Saccharomyces cerevisiae analysis MeSH
- Zymosan analysis isolation & purification MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Zymosan MeSH
Cell walls of the yeast Saccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5 M Na2HPO4, pH 7.8-8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3 h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker's yeast.
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