A new method for isolation of saprophytic cultures of Claviceps fusiformis from sclerotia
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
7106661
DOI
10.1007/bf02877398
Knihovny.cz E-zdroje
- MeSH
- 1-propanol farmakologie MeSH
- Claviceps růst a vývoj izolace a purifikace metabolismus MeSH
- dezinficiencia farmakologie MeSH
- metody MeSH
- námelové alkaloidy biosyntéza MeSH
- resorcinoly farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1-propanol MeSH
- dezinficiencia MeSH
- námelové alkaloidy MeSH
- resorcinoly MeSH
The proposed method includes primary and secondary treatments. Of the various disinfectants tested, 1-propanol and 1-butanol as a primary treatment and phenol, resorcinol, HgC12 or NaCl as a secondary treatment completely eliminated the contamination hazard. 1-Butanol and phenol were not useful disinfectants since they inhibited the growth of C. fusiformis along with the other contaminants. Primary and secondary treatment of sclerotia with 1-propanol and resorcinol, respectively, produced the maximum of stable cultures. Out of 120 cultures tested, 5 cultures demonstrated an appreciable yield of alkaloids under submerged cultural conditions.
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