Since the outbreak of the COVID-19 pandemic, the use of hand sanitisers has become an inseparable part of our personal hygiene. However, the short-term effect and the need for frequent application are shortcomings that impair the overall protection. Another aspect is that repeated use of some products (typically alcohol-based) may cause skin irritation or eventually more severe health problems. This work proposes spray-drying as a suitable method for the preparation of swellable chitosan carriers, allowing for encapsulation and sustained release of antibacterial chlorhexidine digluconate as a model active substance. After application to hands, micron-sized particles preferentially accommodate space between epidermal ridges, protected against attrition. Thanks to their small size (d < 10 μm), particles are comfortable to carry since they are not recognisable by somatosensory receptors. The performance of formulations with various amounts of chlorhexidine and cross-linker was tested and compared with selected commercial disinfectants available on the Czech market (ethanol gel and alcoholic solution with chlorhexidine) against E. coli and S. epidermidis. The real-life performance was investigated with twelve volunteers performing various activities for up to 2 h. Finally, a replica of the human index finger with accurately captured micro-topology was proposed and compared with volunteers' fingers concerning the total amount of adhered and detached particles.
- MeSH
- chlorhexidin MeSH
- dezinfekční prostředky na mytí rukou * MeSH
- Escherichia coli MeSH
- ethanol MeSH
- lidé MeSH
- pandemie MeSH
- prášky, zásypy, pudry MeSH
- ruka mikrobiologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Antimicrobial hand gels have become extremely popular in recent years due to the COVID-19 pandemic. Frequent use of hand sanitising gel can lead to dryness and irritation of the skin. This work focuses on the preparation of antimicrobial acrylic acid (Carbomer)-based gels enhanced by non-traditional compounds-mandelic acid and essential oils-as a substitute for irritating ethanol. Physicochemical properties (pH and viscosity), stability and sensory attributes of the prepared gels were investigated. Antimicrobial activity against representative Gram-positive and Gram-negative bacteria and yeasts was determined. The prepared gels with mandelic acid and essential oil (cinnamon, clove, lemon, and thyme) proved to have antimicrobial activity and even better organoleptic properties than commercial ethanol-based antimicrobial gel. Further, results confirmed that the addition of mandelic acid had a desirable effect on gel properties (antimicrobial, consistency, stability). It has been shown that the essential oil/mandelic acid combination can be a dermatologically beneficial hand sanitiser compared to commercial products. Thus, the produced gels can be used as a natural alternative to alcohol-based daily hand hygiene sanitisers.
- MeSH
- antibakteriální látky MeSH
- antiinfekční látky * MeSH
- COVID-19 * MeSH
- dezinfekční prostředky na mytí rukou * MeSH
- ethanol MeSH
- gely MeSH
- gramnegativní bakterie MeSH
- grampozitivní bakterie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- oleje prchavé * MeSH
- pandemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- dezinfekce MeSH
- dezinficiencia * analýza MeSH
- dezinsekce MeSH
- Publikační typ
- zprávy MeSH
OBJECTIVES: The aim of the study was to investigate possible emergence of resistance to disinfectants in Bordetella pertussis strains isolated from patients with whooping cough in the Czech Republic in 2014 and 2015. METHODS: In an EN1500-based study, clean and dry fingertips of volunteers were always contaminated with one of the two clinical isolates of B. pertussis. Clinical isolates of B. pertussis were obtained from the National Reference Laboratory for Pertussis and Diphtheria, National Institute of Public Health (NIPH), Prague, Czech Republic. Dry and contaminated fingertips were immersed in 10 ml medium and then rubbed with the fingers for 1 minute. After that, the hands were treated with isopropanol 60% v/v or tested products, and then the fingertips were rubbed again into 10 ml of pure medium for 1 minute. The suspensions obtained were immediately diluted and plated on charcoal medium. RESULTS: Ethanol-based product A and propanol-based product B showed bactericidal activity after 30 s of contact. The confidence interval limit for product A and B was 0.12 and 0.19, respectively. Quaternary ammonium compound-based product C was found to be ineffective after 30 s of contact. The confidence interval limit for product C was 0.62. CONCLUSION: Products A and B were assessed as effective against clinical isolates of B. pertussis in accordance with EN 1500. Quaternary ammonium compound-based product C did not comply with the requirements of EN 1500.
- MeSH
- Bordetella pertussis MeSH
- dezinfekční prostředky na mytí rukou * MeSH
- kvartérní amoniové sloučeniny MeSH
- lidé MeSH
- pertuse * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- dezinfekce * metody MeSH
- dezinficiencia MeSH
- lidé MeSH
- ordinace MeSH
- praktické lékařství MeSH
- Check Tag
- lidé MeSH
Microbial biofilm is a major source of problems and microbial contamination across the industrial production, for example in pharmacy, agriculture orfood industry, but also in healthcare. One of the possible solutions to this problem is the inhibition of its formation or eradication of the already formed biofilm. There are many ways to achieve this goal. This review article focuses on the use of chemical disinfectants, antibiotics or biologically active natural substances.
- MeSH
- kontaktní čočky * MeSH
- kyselina hyaluronová fyziologie terapeutické užití MeSH
- lidé MeSH
- oční roztoky MeSH
- roztoky pro kontaktní čočky MeSH
- slzy fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
