Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that regulate plant growth and response to adverse growth conditions, has been mainly focusing on provitamin A biofortification or the production of high-value carotenoids. Here, we show that the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars induced profound metabolic alterations in carotenoid, apocarotenoid and phytohormones pathways. Alterations in isoprenoid- (abscisic acid, gibberellins, cytokinins) and non-isoprenoid (auxin and jasmonic acid) derived hormones together with enhanced xanthophyll content influenced biomass partitioning and abiotic stress tolerance (high light, salt, and drought), and it caused an up to 77% fruit yield increase and enhanced fruit's provitamin A content. In addition, metabolic and hormonal changes led to accumulation of key primary metabolites (e.g. osmoprotectants and antiaging agents) contributing with enhanced abiotic stress tolerance and fruit shelf life. Our findings pave the way for developing a new generation of crops that combine high productivity and increased nutritional value with the capability to cope with climate change-related environmental challenges.
26 stran : ilustrace ; 30 cm
This toolkit intends to inform all International Diabetes Federations (IDF) Members of the IDF call to action on the road to the 2018 United Nations High Level Meeting (HLM) on Non-communicable Diseases, and to provide them with tools to successfully: engage with national policymakers to hold them accountalbe for the implementation of their existing diabetes commitments and demand stronger action to overcome gaps in the years to come; activate the national press to promote the IDF call to action; support the IDF online campaign on the road to the 2018 HLM.
- MeSH
- celosvětové zdraví MeSH
- diabetes mellitus epidemiologie klasifikace patologie MeSH
- epidemiologie MeSH
- komplikace diabetu MeSH
- Konspekt
- Patologie. Klinická medicína
- NLK Obory
- diabetologie
- epidemiologie
- NLK Publikační typ
- informační publikace
140 stran : ilustrace, tabulky ; 24 cm
The publication introduces PACT. Its objective is to ensue the quality of training in ObGyn and of healthcare for women and their babies in Europe. It will help to develope a new European curriculum for training in ObGyn.
- MeSH
- gynekologie výchova MeSH
- kurikulum MeSH
- mezinárodní spolupráce MeSH
- porodnictví výchova MeSH
- studium lékařství specializační postgraduální metody organizace a řízení MeSH
- výchova a vzdělávání MeSH
- Geografické názvy
- Evropa MeSH
- Konspekt
- Výchova a vzdělávání
- NLK Obory
- gynekologie a porodnictví
- výchova a vzdělávání pracovníků ve zdravotnictví
- NLK Publikační typ
- projekty
Health systems and policy analysis Policy brief, ISSN 1997-8065 23
27 stran : ilustrace ; 30 cm
- MeSH
- chronická nemoc MeSH
- integrované poskytování zdravotní péče MeSH
- komorbidita MeSH
- péče orientovaná na pacienta metody MeSH
- Publikační typ
- zprávy MeSH
- Geografické názvy
- Evropa MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- veřejné zdravotnictví
Health systems and policy analysis Policy brief, ISSN 1997-8065 22
28 stran : ilustrace, tabulky ; 30 cm
- MeSH
- chronická nemoc MeSH
- péče orientovaná na pacienta MeSH
- souběh chronických nemocí MeSH
- Publikační typ
- zprávy MeSH
- Geografické názvy
- Evropa MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- veřejné zdravotnictví
Health systems and policy analysis Policy brief, ISSN 1997-8065 26
21 stran : ilustrace, tabulky ; 30 cm
- MeSH
- chronická nemoc MeSH
- integrované poskytování zdravotní péče MeSH
- komorbidita MeSH
- Publikační typ
- zprávy MeSH
- Geografické názvy
- Evropa MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- veřejné zdravotnictví
Health systems and policy analysis Policy brief, ISSN 1997-8065 25
24 stran : ilustrace ; 30 cm
- MeSH
- chronická nemoc MeSH
- komorbidita MeSH
- telemedicína MeSH
- Publikační typ
- zprávy MeSH
- Geografické názvy
- Evropa MeSH
- Konspekt
- Veřejné zdraví a hygiena
- NLK Obory
- veřejné zdravotnictví
Health systems and policy analysis Policy brief, ISSN 1997-8065 24
25 stran : ilustrace, tabulky ; 30 cm
- MeSH
- chronická nemoc ekonomika MeSH
- integrované poskytování zdravotní péče ekonomika MeSH
- komorbidita MeSH
- náklady na zdravotní péči MeSH
- Publikační typ
- zprávy MeSH
- Geografické názvy
- Evropa MeSH
N-linked glycosylation of proteins has both functional and structural significance. Importantly, the glycan structure of a therapeutic protein influences its efficacy, pharmacokinetics, pharmacodynamics and immunogenicity. In this work, we developed glycosylation flux analysis (GFA) for predicting intracellular production and consumption rates (fluxes) of glycoforms, and applied this analysis to CHO fed-batch immunoglobulin G (IgG) production using two different media compositions, with and without additional manganese feeding. The GFA is based on a constraint-based modeling of the glycosylation network, employing a pseudo steady state assumption. While the glycosylation fluxes in the network are balanced at each time point, the GFA allows the fluxes to vary with time by way of two scaling factors: (1) an enzyme-specific factor that captures the temporal changes among glycosylation reactions catalysed by the same enzyme, and (2) the cell specific productivity factor that accounts for the dynamic changes in the IgG production rate. The GFA of the CHO fed-batch cultivations showed that regardless of the media composition, galactosylation fluxes decreased with the cultivation time more significantly than the other glycosylation reactions. Furthermore, the GFA showed that the addition of Mn, a cofactor of galactosyltransferase, has the effect of increasing the galactosylation fluxes but only during the beginning of the cultivation period. The results thus demonstrated the power of the GFA in delineating the dynamic alterations of the glycosylation fluxes by local (enzyme-specific) and global (cell specific productivity) factors.
- MeSH
- CHO buňky MeSH
- Cricetulus MeSH
- galaktosyltransferasy genetika metabolismus MeSH
- glykosylace MeSH
- imunoglobulin G biosyntéza genetika MeSH
- křečci praví MeSH
- rekombinantní proteiny biosyntéza genetika MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- biopsie MeSH
- buněčná inkluze patologie MeSH
- gastritida diagnóza patologie MeSH
- gastrointestinální endoskopie MeSH
- lidé MeSH
- plazmatické buňky patologie MeSH
- senioři MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- dopisy MeSH
- kazuistiky MeSH
- přehledy MeSH
