Cultivation and differentiation of haemopoietic CFU-C cells in semisolid medium
Jazyk angličtina Země Česko Médium print
Typ dokumentu srovnávací studie, časopisecké články
PubMed
7238946
Knihovny.cz E-zdroje
- MeSH
- agar farmakologie MeSH
- analýza kolonii tvořících jednotek * MeSH
- buněčná diferenciace * MeSH
- časové faktory MeSH
- kultivační média MeSH
- kultivované buňky MeSH
- methylcelulosa farmakologie MeSH
- myši MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- agar MeSH
- kultivační média MeSH
- methylcelulosa MeSH
The possibilities of cultivating murine bone marrow in semisolid agar and in methyl cellulose were investigated. The proliferative capacity of the haemopoietic CFU-C stem cells were demonstrated. The growth of CFU-C in fresh murine bone marrow and murine marrow short-term preserved at -75 degrees C was compared. The content of cells capable of proliferation was lower and the onset of colony formation was delayed in preserved bone marrow. Procedures appropriate for routine work were employed for evaluating haemopoietic colonies.
