Disintegration of nuclear envelopes is the only ultrastructural change detectable by freeze-etching in dormant spores of the mould Rhizopus nigricans, both dry and swollen, subjected to repeated freezing and thawing. The increase of the number of freeze-inactivated spores corresponds well with the increase of the number of damaged nuclei. This fact led us to formulate a hypothesis that the structure of the nucleus is the primary target of the freezing or thawing damage. As other biomembranes are not damaged it may be assumed that the disintegration of the nuclear membrane is probably secondary. No changes in ultrastructure of metabolically activated spores could be detected, in spite of the fact that the spores lost their germinative ability. Thus, the mechanism of the freeze injury may be different in dormant and growing spores.

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Folia Microbiol (Praha). 1978;23(2):126-32 PubMed

J Cell Biol. 1963 Jun 1;17(3):609-28 PubMed

Folia Microbiol (Praha). 1963 Sep;40:290-2 PubMed

Cryobiology. 1973 Jun;10(2):157-70 PubMed

Protoplasma. 1973;77(1):15-33 PubMed

J Cell Biol. 1973 Jun;57(3):729-42 PubMed

Biochem Biophys Res Commun. 1975 Jan 20;62(2):308-12 PubMed

Cryobiology. 1976 Jun;13(3):278-86 PubMed