A novel radioimmunoassay of allopregnanolone
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
7618187
DOI
10.1016/0039-128x(94)00039-f
PII: 0039-128X(94)00039-F
Knihovny.cz E-resources
- MeSH
- Chromatography, Liquid MeSH
- Humans MeSH
- Potassium Permanganate MeSH
- Oxidation-Reduction MeSH
- Pregnanolone blood MeSH
- Progesterone isolation & purification MeSH
- Radioimmunoassay methods MeSH
- Reference Values MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Antibody Specificity MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Potassium Permanganate MeSH
- Pregnanolone MeSH
- Progesterone MeSH
A radioimmunoassay for determination of 3 alpha-hydroxy-5 alpha-pregnan-20-one (allopregnanolone) in serum or plasma has been developed and evaluated. The method employs rabbit antiserum to 3 alpha-hydroxy-5 alpha-pregnane-11,20-dione-11-O-carboxymethyloxime bovine serum-albumin conjugate and tritiated radioligand. The main cross-reactant interfering in the assay, progesterone, is eliminated by permanganate oxidation. Two assay variants were compared, with and without a micro-column chromatography. The simplified variant appeared to be reliable enough for determination of allopregnanolone in normally menstruating women at luteal phase, whereas the column-chromatography step is necessary when analyzing samples of expected low analyte concentration as in women in follicular phase, postmenopausal women, or in men. The levels of allopregnanolone in healthy women correlated excellently with progesterone in agreement with previous findings.
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