Cloning of endoglucanase genes from Cellulomonas biazotea into E. coli and S. cerevisiae using shuttle vector YEp24
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
7729760
DOI
10.1007/bf02814307
Knihovny.cz E-zdroje
- MeSH
- bakteriální geny * MeSH
- celulasa genetika MeSH
- Escherichia coli genetika MeSH
- exprese genu MeSH
- genetické vektory MeSH
- geny hub MeSH
- grampozitivní nesporulující tyčinky enzymologie genetika MeSH
- klonování DNA MeSH
- Saccharomyces cerevisiae genetika MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- celulasa MeSH
We constructed a SmaI genomic library of Cellulomonas biazotea DNA in E. coli and in the S. cerevisiae shuttle vector, YEP 24. Three clone were identified that conferred the ability for E. coli or S. cerevisiae transformants to produce carboxymethylcellulase (CMCase). Cells transformed with these clones were compared with one another and with nontransformed cells for hyper-production of CMCase. In vivo and in vitro studies indicated that the CMCase genes were fully expressed and the enzyme activity was located extracellularly. The optimum pH and temperature for the CMCase thus cloned were pH 7 and 50 degrees C, respectively, as was the case for the donor.
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