Characterization of adhesion associated surface properties of uropathogenic Escherichia coli
Language English Country United States Media print
Document type Journal Article
PubMed
7729772
DOI
10.1007/bf02814441
Knihovny.cz E-resources
- MeSH
- Bacterial Adhesion genetics physiology MeSH
- Fimbriae, Bacterial physiology MeSH
- Genes, Bacterial MeSH
- Bacteriuria etiology microbiology MeSH
- Disaccharides chemistry metabolism MeSH
- Child MeSH
- DNA Probes MeSH
- Escherichia coli genetics pathogenicity physiology MeSH
- Hemagglutination MeSH
- Urinary Tract Infections etiology microbiology MeSH
- Escherichia coli Infections etiology microbiology MeSH
- Latex Fixation Tests MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Pyelonephritis etiology microbiology MeSH
- Receptors, Cell Surface metabolism MeSH
- Carbohydrate Sequence MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 4-O-alpha-D-galactopyranosyl-D-galactose MeSH Browser
- Disaccharides MeSH
- DNA Probes MeSH
- galactose receptor MeSH Browser
- Receptors, Cell Surface MeSH
Escherichia coli was isolated from the urine of patients with pyelonephritis, with urinary tract infections other than pyelonephritis and with asymptomatic bacteriuria. Surface properties of the strains were analyzed by the salting-out aggregation test (SAT), hydrophobic interaction chromatography (HIC), Congo red binding (Crb), agglutination of erythrocytes (MRHA) and latex particles covered by digalactoside (PF) and by adherence to tissue culture cells. In addition, a DNA probe for the pap gene was used. The DNA probe detected the highest proportion of strains with pap gene in the group of patients with pyelonephritis, lower in the urinary tract infections other than pyelonephritis and the lowest in the group with asymptomatic bacteriuria. Tests for P-fimbriae (PF, MRHA) showed a similar distribution. Hydrophobicity measured by SAT and by HIC did not show differences among the tested groups of strains. The results suggest that factors other than the P-fimbriae and hydrophobicity may contribute to the persistence of E. coli in the urinary tract.
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