Application of biotinylated and 32P probes for detection of P-fimbriae in urinary E. coli
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
8103498
DOI
10.1007/bf02814388
Knihovny.cz E-zdroje
- MeSH
- bakteriální fimbrie chemie MeSH
- biotin MeSH
- dítě MeSH
- DNA sondy MeSH
- Escherichia coli chemie genetika MeSH
- infekce močového ústrojí mikrobiologie moč MeSH
- infekce vyvolané Escherichia coli mikrobiologie moč MeSH
- lidé MeSH
- mladiství MeSH
- molekulární sekvence - údaje MeSH
- předškolní dítě MeSH
- radioizotopy fosforu MeSH
- sacharidové sekvence MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biotin MeSH
- DNA sondy MeSH
- radioizotopy fosforu MeSH
Escherichia coli is the common causative agent of urinary tract infections. Twenty-six strains of Escherichia coli were isolated from children with pyelonephritis, symptomatic urinary tract infections and asymptomatic bacteriuria. Biotinylated and 32P-DNA probes were prepared for detection of P-fimbriae in the isolates. Of the 13 strains isolated from patients with pyelonephritis 11 were positive for the presence of the P gene by both probes. Strains isolated from cases of symptomatic urinary tract infections revealed the presence of P gene only in three samples of the total of nine isolated. None of the isolated E. coli strains from asymptomatic bacteriuria was found positive for the presence of the P gene. The biotinylated probe was simple and easily applicable in standard laboratory conditions and therefore the authors recommend it for use in diagnostic laboratories.
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