Application of biotinylated and 32P probes for detection of P-fimbriae in urinary E. coli
Language English Country United States Media print
Document type Journal Article
PubMed
8103498
DOI
10.1007/bf02814388
Knihovny.cz E-resources
- MeSH
- Fimbriae, Bacterial chemistry MeSH
- Biotin MeSH
- Child MeSH
- DNA Probes MeSH
- Escherichia coli chemistry genetics MeSH
- Urinary Tract Infections microbiology urine MeSH
- Escherichia coli Infections microbiology urine MeSH
- Humans MeSH
- Adolescent MeSH
- Molecular Sequence Data MeSH
- Child, Preschool MeSH
- Phosphorus Radioisotopes MeSH
- Carbohydrate Sequence MeSH
- Check Tag
- Child MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biotin MeSH
- DNA Probes MeSH
- Phosphorus Radioisotopes MeSH
Escherichia coli is the common causative agent of urinary tract infections. Twenty-six strains of Escherichia coli were isolated from children with pyelonephritis, symptomatic urinary tract infections and asymptomatic bacteriuria. Biotinylated and 32P-DNA probes were prepared for detection of P-fimbriae in the isolates. Of the 13 strains isolated from patients with pyelonephritis 11 were positive for the presence of the P gene by both probes. Strains isolated from cases of symptomatic urinary tract infections revealed the presence of P gene only in three samples of the total of nine isolated. None of the isolated E. coli strains from asymptomatic bacteriuria was found positive for the presence of the P gene. The biotinylated probe was simple and easily applicable in standard laboratory conditions and therefore the authors recommend it for use in diagnostic laboratories.
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