In vitro measurement of translation accuracy of ribosomes isolated from streptomycin-resistant mutant of Streptomyces granaticolor
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
7959428
DOI
10.1007/bf02906807
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence genetika MeSH
- Escherichia coli genetika MeSH
- kinetika MeSH
- mutace MeSH
- proteosyntéza * MeSH
- ribozomy metabolismus MeSH
- Streptomyces účinky léků genetika metabolismus MeSH
- streptomycin farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- streptomycin MeSH
Accuracy of activity of ribosome isolated from UV-light-induced streptomycin-resistant R-21 mutant of Streptomyces granaticolor was measured in an E. coli-derived system translating poly(U) with a high rate and accuracy. Ribosomes from the R-21 mutant strain were shown to be resistant to streptomycin and about two-fold more accurate than those from the wild type. The mutant strain was found to be resistant to 1000 mg/L streptomycin (Stm) during vegetative growth while it sporulated on agar plates containing only up to 200 mg/L of Stm. The growth rate of the R-21 mutant in complex liquid medium was indistinguishable from that of the wild-type strain.
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