Antigens and glycoproteins of larvae, nymphs and adults of the tick Ixodes ricinus
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Antigens analysis MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Glycoproteins analysis MeSH
- Immunoblotting MeSH
- Ticks chemistry growth & development MeSH
- Rabbits immunology MeSH
- Larva MeSH
- Lectins MeSH
- Molecular Weight MeSH
- Organ Specificity MeSH
- Ovary MeSH
- Cross Reactions MeSH
- Animals MeSH
- Check Tag
- Rabbits immunology MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Antigens MeSH
- Glycoproteins MeSH
- Lectins MeSH
Protein components of homogenates of unfed larvae and nymphs of Ixodes ricinus (L.), and of ovary, haemolymph, Malpighian tubules, rectal ampulla, fat body, integument, salivary glands and midgut of partially fed adult females were studied for their antigenicity and carbohydrate moieties using immunoblotting and lectin affinity blotting (LAB) techniques. Comparing the individual anti-larval, anti-nymphal and anti-adult immune sera for their capacity to recognize the specific and trans-stadially cross-reactive antigenic proteins, larval feeding induced the most effective humoral response. The majority of immunogens recognized by rabbit anti-tick immune sera are glycoproteins. Most of the glycosylated antigens were modified with N-type glycans; however, O-type glycans were also demonstrated in some antigens. The correlation of the type of glycosylation with antigenicity, and the sharing of common antigenic epitopes by various tissues, are discussed.
References provided by Crossref.org
A bite so sweet: the glycobiology interface of tick-host-pathogen interactions
