Nystatin changes the properties of transporters for arginine and sugars. An in vitro study
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8062922
DOI
10.1016/0014-5793(94)00730-6
PII: 0014-5793(94)00730-6
Knihovny.cz E-zdroje
- MeSH
- arginin metabolismus MeSH
- biologický transport MeSH
- buněčná membrána metabolismus MeSH
- membránové potenciály MeSH
- metabolismus sacharidů * MeSH
- nystatin farmakologie MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- transportní proteiny metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- arginin MeSH
- nystatin MeSH
- transportní proteiny MeSH
In ergosterol-containing energized yeast plasma membrane vesicles nystatin (5-10 micrograms/mg total lipid) caused a massive efflux of pre-accumulated arginine while the membrane potential (the principal driving force; -110 mV) decreased by only 10-30 mV. Neither the substrate fluxes nor the membrane potential was influenced by nystatin when the permease was reconstituted in ergosterol-free phospholipid vesicles. The same effect of nystatin was found with the reconstituted sugar transporter from Chlorella kessleri. It is suggested that nystatin binding to ergosterol in the vicinity of the permease releases the transport protein from its coupling to energy and converts it to a facilitator.
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General and molecular microbiology and microbial genetics in the IM CAS
