Induction of inflammatory cytokines by Nocardia fractions
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
8206169
Knihovny.cz E-resources
- MeSH
- Fluorescent Antibody Technique MeSH
- Interferon-gamma biosynthesis MeSH
- Interleukins biosynthesis MeSH
- Humans MeSH
- Lipopolysaccharides MeSH
- Swine, Miniature MeSH
- Mitogens immunology MeSH
- Monocytes immunology MeSH
- Nocardia immunology ultrastructure MeSH
- Organic Chemicals MeSH
- Cell Membrane Permeability MeSH
- Swine MeSH
- Cell Wall Skeleton immunology ultrastructure MeSH
- Tumor Necrosis Factor-alpha biosynthesis MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Interferon-gamma MeSH
- Interleukins MeSH
- Lipopolysaccharides MeSH
- Mitogens MeSH
- Nocardia delipidated cell mitogen MeSH Browser
- Organic Chemicals MeSH
- Cell Wall Skeleton MeSH
- Tumor Necrosis Factor-alpha MeSH
NDCM and NLD fractions of Nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. Inflammatory cytokines IL-1, IL-6, tumour necrosis factor (TNF) alpha and interferon (IFN) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. IL-1, IL-6 and TNF were produced by human monocytes stimulated for 2 h, IFN gamma-positive lymphocytes were detected later. IFN gamma was produced also by activated porcine lymphocytes. Cells expressing apoptotic features were found among blood mononuclears treated with Nocardia fractions.
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
Expression of TNF-alpha in pig fetal cells stimulated in vitro
