Induction of inflammatory cytokines by Nocardia fractions
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
8206169
Knihovny.cz E-zdroje
- MeSH
- fluorescenční protilátková technika MeSH
- interferon gama biosyntéza MeSH
- interleukiny biosyntéza MeSH
- lidé MeSH
- lipopolysacharidy MeSH
- miniaturní prasata MeSH
- mitogeny imunologie MeSH
- monocyty imunologie MeSH
- Nocardia imunologie ultrastruktura MeSH
- organické látky MeSH
- permeabilita buněčné membrány MeSH
- prasata MeSH
- skelet buněčné stěny imunologie ultrastruktura MeSH
- TNF-alfa biosyntéza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- interferon gama MeSH
- interleukiny MeSH
- lipopolysacharidy MeSH
- mitogeny MeSH
- Nocardia delipidated cell mitogen MeSH Prohlížeč
- organické látky MeSH
- skelet buněčné stěny MeSH
- TNF-alfa MeSH
NDCM and NLD fractions of Nocardia opaca cell walls were used for in vitro stimulation of human and porcine peripheral blood mononuclear cells. Inflammatory cytokines IL-1, IL-6, tumour necrosis factor (TNF) alpha and interferon (IFN) gamma were detected at the single-cell level using paraformaldehyde-fixed and saponin-permeabilized mononuclears stained with cytokine-specific antibodies and indirect immunofluorescence method. IL-1, IL-6 and TNF were produced by human monocytes stimulated for 2 h, IFN gamma-positive lymphocytes were detected later. IFN gamma was produced also by activated porcine lymphocytes. Cells expressing apoptotic features were found among blood mononuclears treated with Nocardia fractions.
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
Expression of TNF-alpha in pig fetal cells stimulated in vitro
