Inhibition of hepatitis B virus surface gene expression by antisense oligodeoxynucleotides in a human hepatoma cell line
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
8206172
Knihovny.cz E-resources
- MeSH
- Oligonucleotides, Antisense pharmacology MeSH
- Cell Line MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Hepatitis B Surface Antigens genetics metabolism MeSH
- Carcinoma, Hepatocellular microbiology MeSH
- Humans MeSH
- RNA, Messenger metabolism MeSH
- Molecular Sequence Data MeSH
- Gene Expression Regulation, Viral drug effects MeSH
- Base Sequence MeSH
- Genes, Viral drug effects MeSH
- Viral Structural Proteins genetics MeSH
- Hepatitis B virus genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Oligonucleotides, Antisense MeSH
- Hepatitis B Surface Antigens MeSH
- RNA, Messenger MeSH
- Viral Structural Proteins MeSH
We have studied the inhibitory effect of antisense oligodeoxynucleotides on the expression of hepatitis B virus surface antigens. Human hepatoma cell line PLC/PRF/5 harbors several integrated copies of the HBV genome and produces and secretes hepatitis B virus surface antigen (HBsAg) to the medium. Synthetic antisense oligodeoxynucleotides complementary to various regions of the surface antigen gene were synthesized and their ability to block its expression was tested. Oligodeoxynucleotides (17- and 21-mers) complementary to regions covering ATG codons of both preS2 and S genes significantly inhibited preS2 and S protein production. Less efficient inhibition was achieved when the oligonucleotide complementary to the inside S gene region was assayed.
