The effect of purine phosphonomethoxyalkyl derivatives on DNA synthesis in CHO Chinese hamster cells
Language English Country Slovakia Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8289970
Knihovny.cz E-resources
- MeSH
- Adenine analogs & derivatives pharmacology MeSH
- Antiviral Agents pharmacology MeSH
- CHO Cells MeSH
- Cricetulus MeSH
- Cytarabine pharmacology MeSH
- Guanine analogs & derivatives pharmacology MeSH
- Cricetinae MeSH
- Organophosphonates * MeSH
- Organophosphorus Compounds pharmacology MeSH
- DNA Damage MeSH
- DNA Replication drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Zidovudine pharmacology MeSH
- Animals MeSH
- Check Tag
- Cricetinae MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 9-((2-phosphonylmethoxy)ethyl)guanine MeSH Browser
- 9-(2-phosphonylmethoxyethyl)-2,6-diaminopurine MeSH Browser
- 9-(3-hydroxy-2-phosphonomethoxypropyl)guanine MeSH Browser
- 9-(S)-(3-hydroxy-2-(phosphonomethoxy)propyl)adenine MeSH Browser
- adefovir MeSH Browser
- Adenine MeSH
- Antiviral Agents MeSH
- Cytarabine MeSH
- Guanine MeSH
- Organophosphonates * MeSH
- Organophosphorus Compounds MeSH
- Zidovudine MeSH
The inhibition of incorporation of 3H-thymidine and the changes of the rate of nascent DNA chain elongation were investigated in CHO Chinese hamster cells treated with (S)-(3-hydroxy-2-phosphonomethoxypropyl) (HPMP) and N-(2-phosphonomethoxyethyl) (PME) derivatives of adenine (A), guanine (G) and 2,6-diaminopurine (DAP). No direct correlation was observed in PME and HPMP derivatives between cytotoxicity, inhibition of 3H-thymidine incorporation and inhibition of nascent DNA chain elongation. The highest cytotoxicity and inhibition of DNA synthesis were caused by PMEG. The limited extent of inhibition of DNA elongation was encountered in the case of HPMPG and HPMPA. With PMEA, weak inhibition of elongation of DNA was observed only after a prolonged exposure (6 h). None of the investigated drugs induced DNA breaks.
Institute of Experimental Medicine Academy of Sciences of Czech Republic Olesnice v Orlických horách
