Virulence of Naegleria fowleri affected by axenic cultivation and passage in mice
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
8314176
Knihovny.cz E-resources
- MeSH
- Germ-Free Life MeSH
- Mice MeSH
- Naegleria fowleri growth & development pathogenicity MeSH
- Serial Passage MeSH
- Virulence MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
The virulence of Naegleria fowleri decreases with prolonged axenic cultivation. The decline in virulence of highly virulent, mouse-passaged LEE strain amebae was monitored during 5 years of continuous axenic cultivation. The most rapid decrease in virulence occurred during the first 2 years. Virulence could be restored to original levels by 3 serial passages in mice. The composition of axenic media also affected the virulence of N. fowleri, with a more enriched medium appearing to restore some of the virulence of a weakly virulent strain.
