In the series of 212 coagulase-negative staphylococci strains isolated from blood cultures, in which the slime production was tested by means of two methods--a method using agar with Congo red and a Christensen method--S. epidermidis, S. haemolyticus and S. hominis were identified most frequently (in 69.3%, 18.4% and 6.6%, respectively). The slime production was detected by both methods in 28 strains (13%) of four species, most often in S. epidermidis (in 17%). By comparing the Congo red agar method with the Christensen method the sensitivity and specificity of the former was determined (85% and 99%, respectively). Thus the Congo red agar method for the detection of slime production by staphylococci seems to be a reliable tool.

Mikrobiologický ústav lékarské fakulty Masarykovy univerzity a FN u sv Anny Brno

Biofilm detection and the clinical significance of Staphylococcus epidermidis isolates