Functional and structural identification of a new lectin activity of Borrelia recurrentis spirochetes
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Borrelia imunologie MeSH
- galaktosamin farmakologie MeSH
- glukosamin farmakologie MeSH
- glykokonjugáty farmakologie MeSH
- hemaglutinační testy MeSH
- hemaglutininy chemie izolace a purifikace metabolismus MeSH
- hexosaminy farmakologie MeSH
- imunoblotting MeSH
- králíci MeSH
- molekulová hmotnost MeSH
- monoklonální protilátky MeSH
- testy inhibice hemaglutinace MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- galaktosamin MeSH
- glukosamin MeSH
- glykokonjugáty MeSH
- hemaglutininy MeSH
- hexosaminy MeSH
- monoklonální protilátky MeSH
- N-acetylmannosamine MeSH Prohlížeč
1. Haemagglutinating activity (HA) was found and characterized in lysate of the spirochete Borrelia recurrentis. 2. The highest HA was observed using native rabbit red blood cells (RBC), especially oxidized rabbit RBC. 3. In a haemagglutination inhibition test the HA showed an affinity with monosaccharides D-glucosamine, D-galactosamine and N-acetyl-D-mannosamine and several glycoproteins and polysaccharides. 4. An inhibitory effect was also achieved by mouse monoclonal antibody H 9724, polyclonal antibodies from B. recurrentis infected mice and with rabbit polyclonal antibodies directed against B. recurrentis HA. 5. B. recurrentis proteins components of 29, 33, 41 and 55 kDa were identified by immunoblotting as structural subunits of complex binding activity of a new agglutinin.
Citace poskytuje Crossref.org
