Drug-induced changes of rRNA biosynthesis--a marker of toxic damage to embryonal cell population
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article
PubMed
8400452
Knihovny.cz E-resources
- MeSH
- Cell Nucleolus drug effects MeSH
- Cyclophosphamide toxicity MeSH
- Dactinomycin toxicity MeSH
- Erythroid Precursor Cells drug effects MeSH
- Hydrocortisone toxicity MeSH
- Liver drug effects embryology MeSH
- Chick Embryo drug effects MeSH
- DNA Damage * MeSH
- DNA, Ribosomal drug effects genetics MeSH
- RNA, Ribosomal biosynthesis genetics MeSH
- Teratogens toxicity MeSH
- Yolk Sac drug effects MeSH
- Animals MeSH
- Check Tag
- Chick Embryo drug effects MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Cyclophosphamide MeSH
- Dactinomycin MeSH
- Hydrocortisone MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal MeSH
- Teratogens MeSH
The nucleolar test was used for evaluation of the rRNA biosynthesis in two populations of chick embryonic cells. In chick embryos, practically 100 per cent of proerythroblasts (starting from embryonic day 3) as well as hepatocytes (starting with embryonic day 5) synthetized nucleolar, i.e. ribosomal RNA. In consequence of a single administration of the well known teratogens--actinomycin D, cyclophosphamide or hydrocortisone--the percentage of rRNA synthetizing proerythroblasts and hepatocytes decreased characteristically--in dependence on the teratogenic drug. The presented method can be employed when toxicity of drugs or other chemical compounds is evaluated in cell populations possessing high metabolic and proliferative activity.
Development of nucleolar apparatus in the chick primitive erythroid cells
