Early postnatal development of contractile performance and responsiveness to Ca2+, verapamil and ryanodine in the isolated rat heart
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8411198
DOI
10.1006/jmcc.1993.1085
PII: S0022-2828(83)71085-0
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Myocardial Contraction physiology MeSH
- Rats MeSH
- Myocardium ultrastructure MeSH
- Rats, Wistar MeSH
- Ryanodine pharmacology MeSH
- Sarcoplasmic Reticulum physiology ultrastructure MeSH
- Heart anatomy & histology growth & development physiology MeSH
- Aging physiology MeSH
- Calcium pharmacology MeSH
- Calcium Channels physiology MeSH
- Organ Size physiology MeSH
- Verapamil pharmacology MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ryanodine MeSH
- Calcium MeSH
- Calcium Channels MeSH
- Verapamil MeSH
Contractile performance of the isolated perfused rat heart and its inotropic response to Ca2+, verapamil and ryanodine was studied in 1-, 2-, 4-, 7- and 12-day-old animals. Values of the developed force revealed two different phases: a slow decrease from day 1 to day 4 followed by a steep increase up to day 12. A similar biphasic time course was observed in the magnitude of the inotropic effect of Ca2+ (0.6-10.0 mmol.l-1): decrease from day 1 to day 4 followed by an increase up to day 7. The sensitivity to Ca2+ was, however, not changed. An analogous biphasic response was also observed during perfusion with the calcium antagonist verapamil (10(-9) to 3.3 x 10(-7) mol.l-1): the sensitivity to negative inotropic effect rose from day 1 to day 4 and then decreased at day 7 (values of IC50 were 170 +/- 61, 17 +/- 6 and 171 +/- 60 10(-9) mol.l-1 S.E.M. on day 1, 4 and 7, respectively). The contractile response to the inhibitor of calcium release from sarcoplasmic reticulum (SR)--ryanodine (10(-6) mol.l-1)--was surprisingly high already in 1-day-old animals (inhibition of contraction by more than 50%) indicating the presence of functionally active SR in the rat heart just after birth. Our data clearly shows that the early development of contractile function and inotropic responsiveness of the rat heart is not linear and changes dramatically during the first week of life.
References provided by Crossref.org
Sixty Years of Heart Research in the Institute of Physiology of the Czech Academy of Sciences
Developmental Aspects of Cardiac Adaptation to Increased Workload
Neonatal cardiac mitochondria and ischemia/reperfusion injury
Selenium protects the immature rat heart against ischemia/reperfusion injury
