Response of yeast protoplasts to their mating partners
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8768248
DOI
10.1007/bf02818513
Knihovny.cz E-zdroje
- MeSH
- mating faktor MeSH
- peptidy fyziologie MeSH
- protoplasty fyziologie MeSH
- rozmnožování MeSH
- Saccharomyces cerevisiae fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mating faktor MeSH
- peptidy MeSH
The mating process between two protoplasts or between a protoplast and a cell in the yeast Saccharomyces cerevisiae was manifested by a specific morphological response of only the cell partner. The cells produced projections, up to 5 micrometers long, to meet their protoplast partners. The protoplasts responded, after a period of nonspecific hernia-like growth, by ceasing to grow and assuming oval or spherical shapes. They never formed mating projections, apparently due to the absence of complete cell walls. Similarly to the cells, nuclear division in protoplasts was arrested and the nucleus migrated towards the plasma membrane at the site of protoplast-cell contact. Cytoplasmic microtubules were directed to this site, indicating the position of the spindle pole body (SPB) on the nucleus adjacent to the plasma membrane. Actin patches accumulated also in this region. These cytological features of the protoplasts were reminiscent of the reorganization of the cytoskeleton and nucleus characteristic of mating cells. This implies that the ability of protoplasts to produce and receive mating signals was unaffected by protoplasting. Fusion, however, was not initiated due to the absence of the complete cell wall in one of the partners. Thus, the cell wall appeared to be necessary for the expression of polarized growth during mating and for cell fusion.
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Annu Rev Genet. 1993;27:147-79 PubMed
Microbiol Rev. 1992 Mar;56(1):180-94 PubMed
Nature. 1989 Dec 14;342(6251):749-57 PubMed
Mol Cell Biol. 1987 Jul;7(7):2316-28 PubMed
Trends Genet. 1991 Nov-Dec;7(11-12):393-8 PubMed
Arch Microbiol. 1976 Nov 2;110(23):313-8 PubMed
Arch Mikrobiol. 1974 Apr 10;97(1):27-38 PubMed
J Cell Biol. 1990 Oct;111(4):1451-64 PubMed
Mol Cell Biol. 1989 Jun;9(6):2682-94 PubMed
J Cell Sci. 1989 Oct;94 ( Pt 2):207-16 PubMed
Annu Rev Cell Biol. 1988;4:429-57 PubMed
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1304-8 PubMed
Mol Biol Cell. 1994 Nov;5(11):1169-75 PubMed
Cell Biol Int Rep. 1982 Aug;6(8):725-31 PubMed
Mol Biol Cell. 1992 Apr;3(4):429-44 PubMed
Biochim Biophys Acta. 1973 Sep 15;321(1):246-55 PubMed
J Gen Microbiol. 1987 Dec;133(12):3355-63 PubMed
Cell. 1990 Mar 23;60(6):1029-41 PubMed
Methods Cell Biol. 1989;31:357-435 PubMed
