BF3-catalysed methanolysis combined with fast atom bombardment tandem mass spectrometry as a new tool for the preparation and analysis of linear secocyclosporins
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8829477
Knihovny.cz E-resources
- MeSH
- Boranes chemistry MeSH
- Cyclosporins analysis chemical synthesis chemistry MeSH
- Hydrolysis MeSH
- Catalysis MeSH
- Methanol MeSH
- Molecular Sequence Data MeSH
- Oligopeptides chemistry MeSH
- Amino Acid Sequence MeSH
- Spectrometry, Mass, Fast Atom Bombardment MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Boranes MeSH
- boron trifluoride MeSH Browser
- Cyclosporins MeSH
- Methanol MeSH
- Oligopeptides MeSH
BF3-catalysed methanolysis is presented for cyclic peptide cleavage using cyclosporins as model compounds. The reaction conditions for BF3-catalysed methanolysis of cyclosporins were optimized to favour the formation of linear undecapeptides. The resulting secocyclosporins were analysed by fast atom bombardment tandem mass spectrometry. Only one dominant and two side mechanisms of the ring opening are operating so that the complete sequence determination of all prepared oligopeptides was achieved.
Compounds isolated at the Institute of Microbiology in 1989-2001 and future trends
