Location of the alpha-amylase gene in rumen Streptococcus bovis strains distinguished by unstable amylase activity
Language English Country United States Media print
Document type Journal Article
PubMed
8851562
DOI
10.1007/bf02815419
Knihovny.cz E-resources
- MeSH
- alpha-Amylases genetics metabolism MeSH
- Rumen microbiology MeSH
- Bacillus subtilis enzymology genetics MeSH
- Genes, Bacterial * MeSH
- DNA, Bacterial genetics MeSH
- DNA Probes MeSH
- Plasmids genetics MeSH
- Enzyme Stability genetics MeSH
- Streptococcus bovis enzymology genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- alpha-Amylases MeSH
- DNA, Bacterial MeSH
- DNA Probes MeSH
Genetic stability of amylase activity after serial subcultivation experiments with amylolytic ruminal Streptococcus bovis strains was investigated. Two strains Amy+ and Amy- were obtained. Loss of amylase activity connected with the loss of plasmid DNA was not found in these strains. The presence of the gene responsible for the amylase activity in the chromosome of these strains was revealed by hybridization of the alpha-amylase gene on pJK108 against chromosomal DNA of S. bovis and Bacillus subtilis after a complete restriction with EcoRI.
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