Potentiation of GABAA receptor in cultured mouse hippocampal cells by brain-derived peptide mixture cerebrolysin
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8869271
Knihovny.cz E-resources
- MeSH
- 6-Cyano-7-nitroquinoxaline-2,3-dione pharmacology MeSH
- Amino Acids pharmacology MeSH
- Excitatory Amino Acid Antagonists pharmacology MeSH
- Bicuculline pharmacology MeSH
- Dialysis MeSH
- Electrophysiology MeSH
- GABA Antagonists pharmacology MeSH
- gamma-Aminobutyric Acid pharmacology MeSH
- Hippocampus cytology metabolism MeSH
- Cells, Cultured MeSH
- Brain metabolism MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Neurons drug effects physiology MeSH
- Animals, Newborn MeSH
- Receptors, GABA-A drug effects metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 6-Cyano-7-nitroquinoxaline-2,3-dione MeSH
- Amino Acids MeSH
- Excitatory Amino Acid Antagonists MeSH
- Bicuculline MeSH
- cerebrolysin MeSH Browser
- GABA Antagonists MeSH
- gamma-Aminobutyric Acid MeSH
- Receptors, GABA-A MeSH
Application of Cerebrolysin (0.1 microgram per 1 ml) by a fast microperfusion system induced an inward current of 0.2 to 1 nA in all neurones from newborn mouse hippocampi held at -30 mV membrane potential. Cerebrolysin-induced currents were reduced by the GABAA antagonist bicuculline (2 microM) by 65%, by the NMDA antagonist aminophosphovaleric acid (APV, 10 microM) by 27% and by the non-NMDA antagonist cyanonitriquinoxalinedione (CNQX, 10 microM) by 20%. Cerebrolysin dialyzed through a 3.6 kD gut did not induce any transmembrane current but potentiated the response induced by GABA (10 microM) to 135%. We conclude that, in addition to amino acids which activate GABAA, NMDA and non-NMDA receptors, Cerebrolysin also contains a peptide which potentiates the GABAA receptor response.
From Frog Muscle to Brain Neurons: Joys and Sorrows in Neuroscience
