Potentiation of GABAA receptor in cultured mouse hippocampal cells by brain-derived peptide mixture cerebrolysin
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8869271
Knihovny.cz E-zdroje
- MeSH
- 6-kyano-7-nitrochinoxalin-2,3-dion farmakologie MeSH
- aminokyseliny farmakologie MeSH
- antagonisté excitačních aminokyselin farmakologie MeSH
- bikukulin farmakologie MeSH
- dialýza MeSH
- elektrofyziologie MeSH
- GABA antagonisté farmakologie MeSH
- GABA farmakologie MeSH
- hipokampus cytologie metabolismus MeSH
- kultivované buňky MeSH
- mozek metabolismus MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- neurony účinky léků fyziologie MeSH
- novorozená zvířata MeSH
- receptory GABA-A účinky léků metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 6-kyano-7-nitrochinoxalin-2,3-dion MeSH
- aminokyseliny MeSH
- antagonisté excitačních aminokyselin MeSH
- bikukulin MeSH
- cerebrolysin MeSH Prohlížeč
- GABA antagonisté MeSH
- GABA MeSH
- receptory GABA-A MeSH
Application of Cerebrolysin (0.1 microgram per 1 ml) by a fast microperfusion system induced an inward current of 0.2 to 1 nA in all neurones from newborn mouse hippocampi held at -30 mV membrane potential. Cerebrolysin-induced currents were reduced by the GABAA antagonist bicuculline (2 microM) by 65%, by the NMDA antagonist aminophosphovaleric acid (APV, 10 microM) by 27% and by the non-NMDA antagonist cyanonitriquinoxalinedione (CNQX, 10 microM) by 20%. Cerebrolysin dialyzed through a 3.6 kD gut did not induce any transmembrane current but potentiated the response induced by GABA (10 microM) to 135%. We conclude that, in addition to amino acids which activate GABAA, NMDA and non-NMDA receptors, Cerebrolysin also contains a peptide which potentiates the GABAA receptor response.
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