One element, potassium, can be identified as the connecting link in the research of Czech neurophysiologist Prof. František Vyskočil. It accompanied him from the first student experiments on the frog muscle (Solandt effect) via sodium-potassium pump and quantum and non-quantum release of neurotransmitters (e.g. acetylcholine) to the most appreciated work on the reversible leakage of K+ from brain neurons during the Leao´s spreading cortical depression, often preceding migraine. He used a wide range of methods at the systemic, cellular and genetic levels. The electrophysiology and biochemistry of nerve-muscle contacts and synapses in the muscles and brain led to a range of interesting findings and discoveries on normal, denervated and hibernating laboratory mammals and in tissue cultures. Among others, he co-discovered the facilitating effects of catecholamines (adrenaline in particular) by end-plate synchronization of individual evoked quanta. This helps to understand the general effectiveness of nerve-muscle performance during actual stress. After the transition of the Czech Republic to capitalism, together with Dr. Josef Zicha from our Institute, he was an avid promoter of scientometry as an objective system of estimating a scientist´s success in basic research (journal Vesmír, 69: 644-645, 1990 in Czech).

Institute of Physiology of the Czech Academy of Sciences Prague Czech Republic

Zobrazit více v PubMed

Novotný I, Vyskočil F. Inhibition by physostigmine of the increase of oxygen consumption induced by potassium in muscle. Nature. 1961;191:916–917. doi: 10.1038/191916a0. PubMed DOI

Novotný I, Vyskočil F, Vyklický L, Berárdfnek R. Potassium and caffeine induced increase of oxygen consumption in frog muscle and its inhibition by drugs. Physiol Bohemoslov. 1962;11:277–284. PubMed

Novotný I, Vyskočil F, Vyklický L. Effect of drugs on calcium influx in frog sartorius muscle. Biochem Pharmacol. 1963;12:266–268.

Novotný I, Vyskočil F. Der Einfluss von membranstabilisierenden Stoffen aufden Ca45-Einstrom in den Froschmuskel. Pflugers Arch. 1963;278:8. doi: 10.1007/BF00672490. DOI

Novotný I, Vyskočil F. Possible role of Ca ions in the resting metabolism of frog sartorius muscle during potassium depolarization. J Cell Physiol. 1966;67:159–168. doi: 10.1002/jcp.1040670118. PubMed DOI

Beránek R, Vyskočil F. The action of tubocurarine and atropine on the normal and denervated rat diaphragm. J Physiol. 1967;188:53–66. doi: 10.1113/jphysiol.1967.sp008123. PubMed DOI PMC

Beránek R, Vyskočil F. The effect of atropine on the frog sartorius neuromuscular junction. J Physiol. 1968;195:493–503. doi: 10.1113/jphysiol.1968.sp008470. PubMed DOI PMC

Magazanik LG, Vyskočil F. On the possible existence of muscarinic cholinoreceptors on the postsynaptic membrane of the frog muscle. Experientia. 1969;25:606–607. doi: 10.1007/BF01896540. PubMed DOI

Magazanik LG, Vyskočil F. Different action of atropine and some analogues on the end-plate potentials and induced acetylcholine potentials. Experientia. 1969;25:618–619. doi: 10.1007/BF01896548. PubMed DOI

Magazanik LG, Vyskočil F. Dependence of acetylcholine desensitization on the membrane potential of frog muscle fibre and on the ionic changes in the medium. J Physiol. 1970;210:507–518. doi: 10.1113/jphysiol.1970.sp009223. PubMed DOI PMC

Vyskočil F, Magazanik LG. The desensitization of postjunctional muscle membrane after intracellular application of membrane stabilizers and snake venom polypeptides. Brain Res. 1972;48:417–419. doi: 10.1016/0006-8993(72)90202-8. PubMed DOI

Magazanik LG, Vyskočil F. The loci of α-bungarotoxin action on the muscle postjunctional membrane. Brain Res. 1972;48:420–423. doi: 10.1016/0006-8993(72)90203-X. PubMed DOI

Magazanik LG, Vyskočil F. Spontaneous junctional currents in Drosophila muscle-fibers. Physiol Bohemoslov. 1979;28:259–260. PubMed

Vyskočil F, Magazanik LG. Temperature dependence of naja toxin blocking effect in Rana temporaria. Experientia. 1973;29:158–160. doi: 10.1007/BF01945447. PubMed DOI

Magazanik LG, Vyskočil F. The effect of temperature on desensitization kinetics at the post-synaptic membrane of the frog muscle fibre. J Physiol. 1975;249:285–300. doi: 10.1113/jphysiol.1975.sp011016. PubMed DOI PMC

Vyskočil F, Magazanik LG. Dual end-plate potentials at the single neuromuscular junction of the adult frog. Pflugers Arch. 1977;368:271–273. doi: 10.1007/BF00585207. PubMed DOI

Magazanik LG, Nikolsky E, Vyskočil F. Effect of the desensitization-potentiating agent SKF-525a on frog end-plate currents. Eur J Pharmacol. 1982;80:115–119. doi: 10.1016/0014-2999(82)90185-6. PubMed DOI

Magazanik LG, Vyskočil F. Spontaneous junctional currents in Drosophila muscle fibres: effects of temperature, membrane potential and ethanol. Experientia. 1979;35:213–214. doi: 10.1007/BF01920623. PubMed DOI

Magazanik LG, Vyskočil F. Some characteristics of end-plate potentials after partial blockade by α-bungarotoxin in Rana temporaria. Experientia. 1973;29:157–158. doi: 10.1007/BF01945446. PubMed DOI

Vyskočil F, Moravec J, Janský L. Resting state of the myoneural junction in a hibernator. Brain Res. 1971;34:381–384. doi: 10.1016/0006-8993(71)90291-5. PubMed DOI

Moravec J, Melichar I, Janský L, Vyskočil F. Effect of hibernation and noradrenaline on the resting state of neuromuscular junction of golden hamster (Mesocricetus auratus) Pflugers Arch. 1973;345:93–106. doi: 10.1007/BF00585833. PubMed DOI

Melichar I, Brožek G, Janský L, Vyskočil F. Effect of hibernation and noradrenaline on acetylcholine release and action at neuromuscular junction of the golden hamster (Mesocricetus auratus) Pflugers Arch. 1973;345:107–122. doi: 10.1007/BF00585834. PubMed DOI

Vyskočil F, Vyklický L, Huston R. Quantum content at the neuromuscular junction of fast muscle after cross-union with the nerve of slow muscle in the chick. Brain Res. 1971;26:443–445. doi: 10.1016/0006-8993(71)90237-X. PubMed DOI

Ujec E, Vít Z, Vyskočil F, Králík O. Analysis of geometrical and electrical parameters of the tips of glass microelectrodes. Physiol Bohemoslov. 1973;22:329–336. PubMed

Tuček S, Zelená J, Ge I, Vyskočil F. Choline acetyltransferase in transected nerves, denervated muscles and Schwann cells of the frog: correlation of biochemical electron microscopical and electrophysiological observations. Neuroscience. 1978;3:709–724. doi: 10.1016/0306-4522(78)90067-2. PubMed DOI

Vyskočil F, Syrový I, Prusík Z. Induction of extrajunctional acetylcholine sensitivity of rat EDL muscle by peptidic component of peripheral nerve. Pflugers Arch. 1981;390:265–269. doi: 10.1007/BF00658274. PubMed DOI

Vyskočil F, Syrový I. Do peripheral nerves contain a factor inducing acetylcholine sensitivity in skeletal muscle? Experientia. 1979;35:218–219. doi: 10.1007/BF01920626. PubMed DOI

Vyskočil F, Gutmann E. Spontaneous transmitter release from motor nerve endings in muscle fibres of castrated and old animals. Experientia. 1969;25:945–946. doi: 10.1007/BF01898080. PubMed DOI

Gutmann E, Hanzlíková V, Vyskočil F. Age changes in cross striated muscle of the rat. J Physiol. 1971;216:331–343. doi: 10.1113/jphysiol.1971.sp009528. PubMed DOI PMC

Vyskočil F, Gutmann E. Electrophysiological and contractile properties of the levator ani muscle after castration and testosterone administration. Pflugers Arch. 1977;368:105–109. doi: 10.1007/BF01063461. PubMed DOI

Vyskočil F, Gutmann E. Spontaneous transmitter release from nerve endings and contractile properties in the soleus and diaphragm muscles of senile rats. Experientia. 1972;28:280–281. doi: 10.1007/BF01928688. PubMed DOI

Vyskočil F, Gutmann E. Anabolic effect of testosterone on the levator ani muscle of the rat. Pflugers Arch. 1977;371:3–8. doi: 10.1007/BF00580765. PubMed DOI

Vyskočil F, Gutmann E. Control of ACh sensitivity in temporarily unconnected (“decentralized”) segments of diaphragm-muscle fibres of the rat. Pflugers Arch. 1976;367:43–47. doi: 10.1007/BF00583655. PubMed DOI

Vyskočil F, Gutmann E. Contractile and histochemical properties of skeletal-muscles in hibernating and awake golden-hamsters. J Comp Physiol. 1977;122:385–390. doi: 10.1007/BF00692523. DOI

Vyskočil F, Carlson B, Gutmann E. Changes in resting membrane potential and contractility of innervated and denervated skeletal muscle free grafts in the rat. Pflugers Arch. 1973;344:181–186. doi: 10.1007/BF00586551. PubMed DOI

Jones R, Vyskočil F. An electrophysiological examination of the changes in skeletal muscle fibres in response to degenerating nerve tissue. Brain Res. 1975;88:309–317. doi: 10.1016/0006-8993(75)90392-3. PubMed DOI

Vyskočil F, Nikolsky E, Edwards C. An analysis of the mechanisms underlying the non-quantal release of acetylcholine at the mouse neuromuscular junction. Neuroscience. 1983;9:429–435. doi: 10.1016/0306-4522(83)90305-6. PubMed DOI

Lupa MT, Tabti N, Thesleff S, Vyskočil F, Yu SP. The nature and origin of calcium-insensitive miniature end-plate potentials at rodent neuromuscular junctions. J Physiol. 1986;381:607–618. doi: 10.1113/jphysiol.1986.sp016346. PubMed DOI PMC

Thesleff S, Vyskočil F, Ward MR. The action potential in end-plate and extrajunctional regions of rat skeletal muscle. Acta Physiol Scand. 1974;91:196–202. doi: 10.1111/j.1748-1716.1974.tb05676.x. PubMed DOI

Janigro D, Di Gregorio F, Vyskočil F, Gorio A. Gangliosides’ dual mode of action: a working hypothesis. J Neurosci Res. 1984;12:499–509. doi: 10.1002/jnr.490120233. PubMed DOI

Vyskočil F, Di Gregorio F, Gorio A. The facilitating effect of gangliosides on the electrogenic (Na+/K+) pump and on the resistance of the membrane potential to hypoxia in neuromuscular preparation. Pflugers Arch. 1985;403:1–6. doi: 10.1007/BF00583273. PubMed DOI

Walker JL., Jr ion specific liquid ion exchanger microelectrodes. Analyt Chem. 1971;43:90–93. doi: 10.1021/ac60298a780. DOI

Vyskočil F, Kříž N. Modifications of single and double-barrel potassium specific microelectrodes for physiological experiments. Pflugers Arch. 1972;337:365–376. doi: 10.1007/BF00586850. PubMed DOI

Hnik P, Vyskočil F, Kříž N, Holas M. Work-induced increase of extracellular potassium concentration in muscle measured by ion-specific electrodes. Brain Res. 1972;40:559–562. doi: 10.1016/0006-8993(72)90162-X. PubMed DOI

Hník P, Kříž N, Vyskočil F, Smieško V, Mejsnar J, Ujec E, Holas M. Work-induced potassium changes in muscle venous effluent blood measured by ion-specific electrodes. Pflugers Arch. 1973;338:177–181. doi: 10.1007/BF00592752. PubMed DOI

Hník P, Holas M, Krekule I, Kříž N, Mejsnar J, Smieško V, Ujec E, Vyskočil F. Work-induced potassium changes in skeletal muscle and effluent venous blood assessed by liquid ion-exchanger microelectrodes. Pflugers Arch. 1976;362:85–94. doi: 10.1007/BF00588685. PubMed DOI

Hník P, Kříž N, Vyskočil F, Smieško V, Mejsnar J, Ujec E, Holas M. Work-induced increase of potassium in venous effluent blood measured by ion-specific electrodes. Physiol Bohemoslov. 1973;22:67–68. doi: 10.1007/BF00592752. PubMed DOI

Vyskočil F, Hník P, Rehfeldt H, Vejsada R, Ujec E. The measurement of K+e concentration changes in human muscles during volitional contractions. Pflugers Arch. 1983;399:235–237. doi: 10.1007/BF00656721. PubMed DOI

Vyskočil F, Kříž N, Bureš J. Potassium-selective microelectrodes used for measuring the extracellular brain potassium during spreading depression and anoxic depolarization in rats. Brain Res. 1972;39:255–259. doi: 10.1016/0006-8993(72)90802-5. PubMed DOI

Adámek S, Vyskočil F. Potassium-selective microelectrode revealed difference in threshold potassium concentration for cortical spreading depression in female and male rat brain. Brain Res. 2011;1370:215–219. doi: 10.1016/j.brainres.2010.11.018. PubMed DOI

Vizi ES, Vyskočil F. Changes in total and quantal release of acetylcholine in the mouse diaphragm during activation and inhibition of membrane ATPase. J Physiol. 1979;286:1–14. doi: 10.1113/jphysiol.1979.sp012603. PubMed DOI PMC

Vyskočil F, Illés P. Non-quantal release of transmitter at mouse neuromuscular junction and its dependence on the activity of Na+-K+ ATP-ase. Pflugers Arch. 1977;370:295–297. doi: 10.1007/BF00585542. PubMed DOI

Vyskočil F, Illés P. Electrophysiological examination of transmitter release in non-quantal form in the mouse diaphragm and the activity of membrane ATP-ase. Physiol Bohemoslov. 1978;27:449–455. PubMed

Edwards C, Doležal V, Tuček S, Zemková H, Vyskočil F. Is an acetylcholine transport system responsible for nonquantal release of acetylcholine at the rodent myoneural junction? Proc Natl Acad Sci U S A. 1985;82:3514–3518. doi: 10.1073/pnas.82.10.3514. PubMed DOI PMC

Vyskočil F. Inhibition of non-quantal acetylcholine leakage by 2(4-phenylpiperidine)cyclohexanol in the mouse diaphragm. Neurosci Lett. 1985;59:277–280. doi: 10.1016/0304-3940(85)90144-2. PubMed DOI

Zemková H, Vyskočil F, Edwards C. The effects of nerve terminal activity on non-quantal release of acetylcholine at the mouse neuromuscular junction. J Physiol. 1990;423:631–640. doi: 10.1113/jphysiol.1990.sp018044. PubMed DOI PMC

Nikolsky EE, Voronin VA, Oranska TI, Vyskočil F. The dependence of non-quantal acetylcholine release on the choline-uptake system in the mouse diaphragm. Pflügers Arch. 1991;418:74–78. doi: 10.1007/BF00370454. PubMed DOI

Zemková H, Vyskočil F, Edwards C. A study on early post-denervation changes of non-quantal and quantal acetylcholine release in the rat diaphragm. Pflugers Arch. 1987;409:540–546. doi: 10.1007/BF00583813. PubMed DOI

Zemková H, Vyskočil F. Effect of Mg2+ on non-quantal acetylcholine release at the mouse neuromuscular junction. Neurosci Lett. 1989;103:293–297. doi: 10.1016/0304-3940(89)90115-8. PubMed DOI

Nikolsky EE, Voronin VA, Vyskočil F. Kinetic differences in the effect of calcium on quantal and non-quantal acetylcholine release at the murine diaphragm. Neurosci Lett. 1991;123:192–194. doi: 10.1016/0304-3940(91)90928-M. PubMed DOI

Nikolsky EE, Oranska TI, Vyskočil F. Non-quantal acetylcholine release after cholinesterase inhibition in vivo. Physiol Res. 1992;41:333–334. PubMed

Giniatullin RA, Khazipov RN, Oranska TI, Nikolsky EE, Voronin VA, Vyskočil F. The effect of non-quantal acetylcholine release on quantal miniature currents at mouse diaphragm. J Physiol. 1993;466:105–114. doi: 10.1113/jphysiol.1993.sp019711. PubMed DOI PMC

Giniatullin RA, Khazipov RN, Vyskočil F. A correlation between quantal content and decay time of endplate currents in frog muscles with intact cholinesterase. J Physiol. 1993;466:95–103. doi: 10.1113/jphysiol.1993.sp019710. PubMed DOI PMC

Nikolsky EE, Zemková H, Voronin VA, Vyskočil F. Role of non-quantal acetylcholine release in surplus polarization of mouse diaphragm fibres at the endplate zone. J Physiol. 1994;477:497–502. doi: 10.1113/jphysiol.1994.sp020210. PubMed DOI PMC

Vyskočil F, Vrbová G. Non-quantal release of acetylcholine affects polyneuronal innervation on developing rat muscle fibres. Eur J Neurosci. 1993;5:1677–1683. doi: 10.1111/j.1460-9568.1993.tb00235.x. PubMed DOI

Shakiryanova DM, Zefirov AL, Nikolsky EE, Vyskočil F. The effect of acetylcholine and related drugs on currents at the frog motor nerve terminal. Eur J Pharmacol. 1994;263:107–114. doi: 10.1016/0014-2999(94)90530-4. PubMed DOI

Vyskočil F, Malomouzh AI, Nikolsky EE. Non-quantal acetylcholine release at the neuromuscular junction. Physiol Res. 2009;58:763–784. doi: 10.33549/physiolres.931865. PubMed DOI

Nikolsky EE, Oranska TI, Vyskočil F. Non-quantal acetylcholine release in the mouse diaphragm after phrenic nerve crush and during recovery. Exp Physiol. 1996;81:341–348. doi: 10.1113/expphysiol.1996.sp003938. PubMed DOI

Mukhtarov MR, Urazaev AK, Nikolsky EE, Vyskočil F. Effect of nitric oxide and NO synthase inhibition on nonquantal acetylcholine release in the rat diaphragm. Eur J Neurosci. 2000;12:980–986. doi: 10.1046/j.1460-9568.2000.00992.x. PubMed DOI

Galkin AV, Giniatullin RA, Mukhtarov MR, Svandová I, Grishin SN, Vyskočil F. ATP but not adenosine inhibits nonquantal acetylcholine release at the mouse neuromuscular junction. Eur J Neurosci. 2001;13:2047–2053. doi: 10.1046/j.0953-816x.2001.01582.x. PubMed DOI

Teplov AY, Grishin SN, Mukhamedyarov MA, Ziganshin AU, Zefirov AL, Palotás A. Ovalbumin-induced sensitization affects non-quantal acetylcholine release from motor nerve terminals and alters contractility of skeletal muscles in mice. Exp Physiol. 2009;94:264–268. doi: 10.1113/expphysiol.2008.045740. PubMed DOI

Malomouzh AI, Nikolsky EE, Vyskočil F. Purine P2Y receptors in ATP-mediated regulation of non-quantal acetylcholine release from motor nerve endings of rat diaphragm. Neurosci Res. 2011;71:219–225. doi: 10.1016/j.neures.2011.07.1829. PubMed DOI

Giniatullin RA, Talantova M, Vyskočil F. Desensitization shortens the high-quantal-content endplate current time course in frog muscle with intact cholinesterase. J Physiol. 1997;502:641–648. doi: 10.1111/j.1469-7793.1997.641bj.x. PubMed DOI PMC

Lindovský J, Kaniaková M, Svobodová L, Vyskočil F, Krůšek J. Role of negatively charged amino acids in beta 4 F-loop in activation and desensitization of alpha 3 beta 4 rat neuronal nicotinic receptors. Biochim Biophys Acta. 2008;1778:864–871. doi: 10.1016/j.bbamem.2008.01.010. PubMed DOI

Kaniaková M, Lindovský J, Krůšek J, Adámek S, Vyskočil F. Dual effect of lobeline on α4β2 rat neuronal nicotinic receptors. Eur J Pharmacol. 2011;658:108–113. doi: 10.1016/j.ejphar.2011.02.012. PubMed DOI

Lindovský J, Petrov K, Krůšek J, Reznik VS, Nikolsky EE, Vyskočil F. Effect of tissue-specific acetylcholinesterase inhibitor C-547 on α3β4 and αβεδ acetylcholine receptors in COS cells. Eur J Pharmacol. 2012;688:22–26. doi: 10.1016/j.ejphar.2012.05.010. PubMed DOI

Skorinkin AI, Shaihutdinova AR, Vyskočil F. Model of concentration changes across the synaptic cleft during a single quantum release. Gen Physiol Biophys. 2008;27:19–24. PubMed

Gilmanov IR, Samigullin DV, Vyskočil F, Nikolsky EE, Bukharaeva EA. Modeling of quantal neurotransmitter release kinetics in the presence of fixed and mobile calcium buffers. J Comput Neurosci. 2008;25:296–307. doi: 10.1007/s10827-008-0079-5. PubMed DOI

Adámek S, Shakirzyanova AV, Malomouzh AI, Naumenko NV, Vyskočil F. Interaction of glutamate- and adenosine-induced decrease of acetylcholine quantal release at frog neuromuscular junction. Physiol Res. 2010;59:803–810. doi: 10.33549/physiolres.932024. PubMed DOI

Urazaev AK, Magsumov ST, Poletayev GI, Nikolsky EE, Vyskočil F. Muscle NMDA receptors regulate the resting membrane potential through NO-synthase. Physiol Res. 1995;44:205–208. PubMed

Mukhtarov MR, Vyskočil F, Urazaev AK, Nikolsky EE. Non-quantal acetylcholine release is increased after nitric oxide synthase inhibition. Physiol Res. 1999;48:315–317. PubMed

Urazaev A, Nikolsky E, Vyskočil F. Muscarinic M-1-receptors are involved in neural regulation of membrane potential in rat muscles. J Neurochem. 1998;71(Suppl 1):S22.

Urazaev AKh, Naumenko NV, Poletayev GI, Nikolsky EE, Vyskočil F. The effect of glutamate and inhibitors of NMDA receptors on postdenervation decrease of membrane potential in rat diaphragm. Mol Chem Neuropathol. 1998;33:163–174. doi: 10.1007/BF02815179. PubMed DOI

Urazaev AKh, Naumenko NV, Poletayev GI, Nikolsky EE, Vyskočil F. Nitroprusside decreases the early post-denervation depolarization of diaphragm muscle fibres of the rat. Eur J Pharmacol. 1996;316:219–222. doi: 10.1016/S0014-2999(96)00817-5. PubMed DOI

Urazaev AKh, Naumenko NV, Nikolsky EE, Vyskočil F. Carnosine and other imidazole-containing compounds enhance the postdenervation depolarization of the rat diaphragm fibres. Physiol Res. 1998;47:291–295. PubMed

Moravec J, Vyskočil F. Early postdenervation depolarization develops faster at endplates of hibernating golden hamsters where spontaneous quantal and non-quantal acetylcholine release is very small. Neurosci Res. 2005;51:25–29. doi: 10.1016/j.neures.2004.09.003. PubMed DOI

Urazaev AK, Naumenko NV, Poletayev GI, Nikolsky EE, Vyskočil F. Acetylcholine and carbachol prevent muscle depolarization in denervated rat diaphragm. Neuroreport. 1997;8:403–406. doi: 10.1097/00001756-199701200-00004. PubMed DOI

Urazaev AK, Naumenko NV, Nikolsky EE, Vyskočil F. The glutamate and carbachol effects on the early post-denervation depolarization in rat diaphragm are directed towards furosemide-sensitive chloride transport. Neurosci Res. 1999;33:81–86. doi: 10.1016/S0168-0102(98)00117-5. PubMed DOI

Malomouzh AI, Arkhipova SS, Nikolsky EE, Vyskočil F. Immunocytochemical demonstration of M(1) muscarinic acetylcholine receptors at the presynaptic and postsynaptic membranes of rat diaphragm endplates. Physiol Res. 2011;60:185–188. doi: 10.33549/physiolres.932131. PubMed DOI

Samigullin D, Bukharaeva EA, Nikolsky E, Adámek S, Vyskočil F. Long release latencies are increased by acetylcholine at frog endplate. Physiol Res. 2003;52:475–480. doi: 10.33549/physiolres.930407. PubMed DOI

Bukcharaeva EA, Kim KC, Moravec J, Nikolsky EE, Vyskočil F. Noradrenaline synchronizes evoked quantal release at frog neuromuscular junctions. J Physiol. 1999;517:879–888. doi: 10.1111/j.1469-7793.1999.0879s.x. PubMed DOI PMC

Bukharaeva E, Nikolsky E, Kim K, Gainulov R, Vyskočil F. Catecholamines change the time course of the evoked quanta mediator release via beta(1)-adrenoreceptors. J Neurochem. 1998;71(Suppl 1):S49.

Bukharaeva EA, Samigullin D, Nikolsky E, Vyskočil F. Protein kinase A cascade regulates quantal release dispersion at frog muscle endplate. J Physiol. 2002;538:837–848. doi: 10.1113/jphysiol.2001.012752. PubMed DOI PMC

Khuzakhmetova V, Bukharaeva E. Adrenaline facilitates synaptic transmission by synchronizing release of acetylcholine quanta from motor nerve endings. Cell Mol Neurobiol. 2021;41:395–401. doi: 10.1007/s10571-020-00840-3. PubMed DOI PMC

Ujec E, Vyklický L, Jr, Vyskočil F, Vyklický L., Sr A simple version of the patch clamp technique for measuring currents in single ionic channels of biological-membranes. Physiol Bohemoslov. 1984;33:570–571.

Zemková H, Vyskočil F, Tolar M, Vlachová V, Ujec E. Single K+ currents during differentiation of embryonic muscle cells in vitro. Biochim Biophys Acta. 1989;986:146–150. doi: 10.1016/0005-2736(89)90284-8. PubMed DOI

Bolotina V, Borecký J, Vlachová V, Baudyšová M, Vyskočil F. Voltage-dependent chloride channels with several substates in excised patches from mouse neuroblastoma cells. Neurosci Lett. 1987;77:298–302. doi: 10.1016/0304-3940(87)90516-7. PubMed DOI

Vyklický L, Jr, Michl J, Vlachová V, Vyklický L, Vyskočil F. Ionic currents in neuroblastoma clone E-7 cells. Neurosci Lett. 1985;55:197–201. doi: 10.1016/0304-3940(85)90019-9. PubMed DOI

Krůšek J, Dittert I, Hendrych T, Hník P, Horák M, Petrovic M, Sedláček M, Sušánková K, Svobodová L, Toušová K, Ujec E, Vlachová V, Vyklický L, Vyskočil F, Vyklický L., Jr Activation and modulation of ligand-gated ion channels. Physiol Res. 2004;53(Suppl 1):S103–S113. doi: 10.33549/physiolres.930000.53.S103. PubMed DOI

Vlachová V, Vyklický L, Vyklický L, Jr, Vyskočil F. The action of excitatory amino acids on chick spinal cord neurones in culture. J Physiol. 1987;386:425–438. doi: 10.1113/jphysiol.1987.sp016542. PubMed DOI PMC

Vyklický L, Vyklický L, Jr, Vyskočil F, Vlachová V, Ujec E, Michl J. Evidence that excitatory amino acids not only activate the receptor channel complex but also lead to use-dependent block. Brain Res. 1986;363:148–151. doi: 10.1016/0006-8993(86)90668-2. PubMed DOI

Zemková H, Krůšek J, Vyskočil F. Potentiation of GABAA receptor in cultured mouse hippocampal cells by brain-derived peptide mixture cerebrolysin. Physiol Res. 1995;44:151–155. PubMed

Vyklický L, Vyklický L, Jr, Vlachová V, Michl J, Vyskočil F. Cobalt ions block L-glutamate and L-aspartate-induced currents in cultured neurons from embryonic chick spinal cord. Neurosci Lett. 1985;61:345–350. doi: 10.1016/0304-3940(85)90488-4. PubMed DOI

Vyskočil F, Vyklický L, Vlachová V, Ujec E. Miniature endplate currents at the neuromuscular-junction of the larva of the fly (Sarcophaga bullata) Mol Physiol. 1985;7:143–154.

Vyklický L, Vyklický L, Vlachová V, Vyskočil F, Michl J. Action of L-glutamate and L-aspartate on spinal cord neurons in cell culture. Physiol Bohemoslov. 1986;35:375.

Hamplová-Peichlová J, Krůšek J, Paclt I, Slavíček J, Lisá V, Vyskočil F. Citalopram inhibits L-type calcium channel current in rat cardiomyocytes in culture. Physiol Res. 2002;51:317–321. doi: 10.33549/physiolres.930216. PubMed DOI

Krůšek J, Vyskočil F. Different degree of cooperativity in adult, embryonic and mutated mouse muscle nicotinic receptors. Biochim Biophys Acta. 2003;1646:119–130. doi: 10.1016/S1570-9639(02)00552-6. PubMed DOI

Giniatullin RA, Kheeroug LS, Vyskočil F. Modelling endplate currents: dependence on quantum secretion probability and decay of miniature current. Eur Biophys J. 1995;23:443–446. doi: 10.1007/BF00196832. PubMed DOI

Shaihutdinova AR, Nikolsky EE, Vyskočil F, Skorinkin AI. Mechanisms of the inhibition of endplate acetylcholine receptors by antiseptic chlorhexidine (experiments and models) Naunyn Schmiedebergs Arch Pharmacol. 2009;380:551–560. doi: 10.1007/s00210-009-0458-0. PubMed DOI

Giniatullin RA, Talantova MV, Vyskočil F. The role of desensitisation in decay time of miniature endplate currents in frogs Rana ridibunda and Rana temporaria. Neurosci Res. 2001;39:287–292. doi: 10.1016/S0168-0102(00)00225-X. PubMed DOI

Strunsky EG, Borisover MD, Nikolsky EE, Vyskočil F. Temperature effect on carbachol-induced depression of spontaneous quantal transmitter release in frog neuromuscular junction. Neurochem Res. 2001;26:891–897. doi: 10.1023/A:1012372115058. PubMed DOI

Kovyazina IV, Nikolsky EE, Giniatullin RA, Adámek S, Vyskočil F. Dependence of miniature endplate current on kinetic parameters of acetylcholine receptors activation: a model study. Neurochem Res. 2003;28:443–448. doi: 10.1023/A:1022896601271. PubMed DOI

Dlouhá H, Teisinger J, Vyskočil F. Activation of membrane Na+/K+-ATPase of mouse skeletal muscle by acetylcholine and its inhibition by alpha-bungarotoxin, curare and atropine. Pflugers Arch. 1979;380:101–104. doi: 10.1007/BF00582620. PubMed DOI

Dlouhá H, Teisinger J, Vyskočil F. The effect of vanadate on the electrogenic Na+/K+ pump, intracellular Na+ concentration and electrophysiological characteristics of mouse skeletal muscle fibre. Physiol Bohemoslov. 1981;30:1–10. PubMed

Vyskočil F, Teisinger J, Dlouhá H. A specific enzyme is not necessary for vanadate-induced oxidation of NADH. Nature. 1980;286:516–517. doi: 10.1038/286516a0. PubMed DOI

Amler E, Teisinger J, Svobodová J, Vyskočil F. Vanadyl ions increase the order parameter of plasma membranes without changing the rotational relaxation time. Biochim Biophys Acta. 1986;863:18–22. doi: 10.1016/0005-2736(86)90382-2. PubMed DOI

Stankovičová T, Zemková H, Breier A, Amler E, Burkhard M, Vyskočil F. The effects of calcium and calcium channel blockers on sodium pump. Pflugers Arch. 1995;429:716–721. doi: 10.1007/BF00373994. PubMed DOI

Amler E, Teisinger J, Svoboda P, Vyskočil F. The changes in conformation of (Na+ K+)-ATPase from rat brain membranes are accompanied by changes of protein segment movements in the nanosecond range. Physiol Bohemoslov. 1988;37:145–148. PubMed

Sulová Z, Vyskočil F, Stankovičová T, Breier A. Ca2+-induced inhibition of sodium pump: effects on energetic metabolism of mouse diaphragm tissue. Gen Physiol Biophys. 1998;17:271–283. PubMed

Zemková H, Teisinger J, Vyskočil F. Inhibition of the electrogenic Na,K pump and Na,K-ATPase activity by tetraethylammonium, tetrabutylammonium, and apamin. J Neurosci Res. 1988;19:497–503. doi: 10.1002/jnr.490190414. PubMed DOI

Vyskočil P, Zemková H, Teisinger J, Vyskočil F. Arachidonate has a positive effect on the electrogenic sodium-potassium pump in the mouse diaphragm. Physiol Bohemoslov. 1987;36:167–169. PubMed

Vyskočil F, Zemková H, Teisinger J, Svoboda P. Arachidonate activates muscle electrogenic sodium pump and brain microsome Na+,K+-ATPase under suboptimal conditions. Brain Res. 1987;436:85–91. doi: 10.1016/0006-8993(87)91559-9. PubMed DOI

Svoboda P, Teisinger J, Vyskočil F. Effect of catecholamines and metal chelating agents on the brain and brown adipose tissue Na,K-ATPase. Comp Biochem Physiol C Comp Pharmacol. 1986;84:283–290. doi: 10.1016/0742-8413(86)90095-2. PubMed DOI

Tyapkina O, Volkov E, Nurullin L, Shenkman B, Kozlovskaya I, Nikolsky E, Vyskočil F. Resting membrane potential and Na+,K+-ATPase of rat fast and slow muscles during modeling of hypogravity. Physiol Res. 2009;58:599–603. doi: 10.33549/physiolres.931810. PubMed DOI

Khuzakhmetova V, Samigullin D, Nurullin L, Vyskočil F, Nikolsky E, Bukharaeva E. Kinetics of neurotransmitter release in neuromuscular synapses of newborn and adult rats. Int J Dev Neurosci. 2014;34:9–18. doi: 10.1016/j.ijdevneu.2013.12.010. PubMed DOI

Bukharaeva E, Ipatova T, Nikolsky EE, Vyskočil F. The effect of carbachol and alpha-bungarotoxin on the frequency of miniature endplate potentials at the frog neuromuscular junction. Exp Physiol. 2000;85:125–131. doi: 10.1017/S0958067000019497. PubMed DOI

Doležal V, Vyskočil F, Tuček S. Decrease of the spontaneous non-quantal release of acetylcholine from the phrenic nerve in botulinum-poisoned rat diaphragm. Pflugers Arch. 1983;397:319–322. doi: 10.1007/BF00580268. PubMed DOI

Samigullin D, Bukharaeva E, Nikolsky E, Vyskočil F. Temperature effect on proximal to distal gradient of quantal release of acetylcholine at frog endplate. Neurochem Res. 2003;28:507–514. doi: 10.1023/A:1022817205814. PubMed DOI

Vyskočil F. Early postdenervation depolarization is controlled by acetylcholine and glutamate via nitric oxide regulation of the chloride transporter. Neurochem Res. 2003;28:575–585. doi: 10.1023/A:1022833709448. PubMed DOI

Malomouzh AI, Mukhtarov MR, Nikolsky EE, Vyskočil F, Lieberman EM, Urazaev AK. Glutamate regulation of non-quantal release of acetylcholine in the rat neuromuscular junction. J Neurochem. 2003;85:206–213. doi: 10.1046/j.1471-4159.2003.01660.x. PubMed DOI

Volkov EM, Nurullin LF, Nikolsky E, Krůšek J, Vyskočil F. Chloride cotransport in the membrane of earthworm body wall muscles. Physiol Res. 2003;52:587–592. doi: 10.33549/physiolres.930182. PubMed DOI

Nikolsky EE, Vyskočil F, Bukharaeva EA, Samigullin D, Magazanik LG. Cholinergic regulation of the evoked quantal release at frog neuromuscular junction. J Physiol. 2004;560:77–88. doi: 10.1113/jphysiol.2004.065805. PubMed DOI PMC

Svobodová L, Krůšek J, Hendrych T, Vyskočil F. Allosteric modulation of nicotinic acetylcholine receptor by physostigmine. Biophysics from Molecules to Brain: In Memory of Radoslav K. Andjus. 2005;1048:251–255. PubMed

Bukharaeva EA, Salakhutdinov RI, Vyskočil F, Nikolsky EE. Spontaneous quantal and non-quantal release of acetylcholine at mouse endplate during onset of hypoxia. Physiol Res. 2005;54:251–255. doi: 10.33549/physiolres.930718. PubMed DOI

Samigullin D, Bukharaeva EA, Vyskočil F, Nikolsky EE. Calcium dependence of uni-quantal release latencies and quantal content at mouse neuromuscular junction. Physiol Res. 2005;54:129–132. doi: 10.33549/physiolres.930712. PubMed DOI

Petrov KA, Kovyazina LV, Zobov VV, Bukharaeva EA, Nikolsky EE, Vyskočil F. Different sensitivity of miniature endplate currents of the rat extensor digitorum longus, soleus and diaphragm muscles to a novel acetylcholinesterase inhibitor C-547. Physiol Res. 2006;55:585–589. doi: 10.33549/physiolres.930980. PubMed DOI

Svobodová L, Krůšek J, Hendrych T, Vyskočil F. Physostigmine modulation of acetylcholine currents in COS cells transfected with mouse muscle nicotinic receptor. Neurosci Lett. 2006;401:20–24. doi: 10.1016/j.neulet.2006.02.065. PubMed DOI

Malomouzh AI, Mukhtarov MR, Nikolsky EE, Vyskočil F. Muscarinic M1 acetylcholine receptors regulate the non-quantal release of acetylcholine in the rat neuromuscular junction via NO-dependent mechanism. J Neurochem. 2007;102:2110–2117. doi: 10.1111/j.1471-4159.2007.04696.x. PubMed DOI

Volkov EM, Nurullin LF, Nikolsky E, Vyskočil F. Miniature excitatory synaptic ion currents in the earthworm Lumbricus terrestris body wall muscles. Physiol Res. 2007;56:655–658. doi: 10.33549/physiolres.931269. PubMed DOI

Volkov EM, Nurullin LF, Volkov ME, Nikolsky EE, Vyskočil F. Mechanisms of carbacholine and GABA action on resting membrane potential and Na+/K+-ATPase of Lumbricus terrestris body wall muscles. Comp Biochem Physiol A Mol Integr Physiol. 2011;158:520–524. doi: 10.1016/j.cbpa.2010.12.016. PubMed DOI

Nurullin LF, Mukhitov AR, Tsentsevytsky AN, Petrova NV, Samigullin DV, Malomouzh AI, Bukharaeva EA, Vyskočil F, Nikolsky EE. Voltage-dependent P/Q-type calcium channels at the frog neuromuscular junction. Physiol Res. 2011;60:815–823. doi: 10.33549/physiolres.932219. PubMed DOI

Petrov KA, Yagodina LO, Valeeva GR, Lannik NI, Nikitashina AD, Rizvanov AA, Zobov VV, Bukharaeva EA, Reznik VS, Nikolsky EE, Vyskočil F. Different sensitivities of rat skeletal muscles and brain to novel anti-cholinesterase agents, alkylammonium derivatives of 6-methyluracil (ADEMS) Br J Pharmacol. 2011;163:732–744. doi: 10.1111/j.1476-5381.2011.01211.x. PubMed DOI PMC

Vyskočil F. Action potentials of the rat diaphragm and their sensitivity to tetrodotoxin during postnatal development and old age. Pflugers Arch. 1974;352:155–163. doi: 10.1007/BF00587514. PubMed DOI

Kaniakova M, Skrenkova K, Adamek S, Vyskocil F, Krusek J. Different effects of lobeline on neuronal and muscle nicotinic receptors. Eur J Pharmacol. 2014;738:352–359. doi: 10.1016/j.ejphar.2014.05.057. PubMed DOI

Vyskočil F. Recovery of sensitivity to acetylcholine following desensitization in muscles of different vertebrate species. Pflugers Arch. 1975;361:83–87. doi: 10.1007/BF00587345. PubMed DOI

Vyskočil F. Miniature end-plate potentials and sensitivity to acetylcholine in the fast and slow limb muscles of hibernating golden hamsters. Pflugers Arch. 1976;361:165–167. doi: 10.1007/BF00583461. PubMed DOI

Vyskočil F. Diazepam blockade of repetitive action potentials in skeletal muscle fibres. A model of its membrane action. Brain Res. 1977;133:315–328. doi: 10.1016/0006-8993(77)90767-3. PubMed DOI

Vyskočil F. Effect of diazepam on the frog neuromuscular junction. Eur J Pharmacol. 1978;48:117–124. doi: 10.1016/0014-2999(78)90049-3. PubMed DOI

Illés P, Vyskočil F. Calcium-dependent inhibition by prostaglandin E1 of spontaneous acetylcholine release from frog motor nerve. Eur J Pharmacol. 1978;48:455–457. doi: 10.1016/0014-2999(78)90175-9. PubMed DOI

Dlouhá H, Vyskočil F. The effect of cortisol on the excitability of the rat muscle fibre membrane and neuromuscular transmission. Physiol Bohemoslov. 1979;28:485–494. PubMed

Vejsada R, Hník P, Dittertová-Vlasáková L, Smetana K, Jr, Haninec P, Grim M, Vyskočil F. Functional properties of muscle autografts substituted for the rat levator ani muscle. Physiol Bohemoslov. 1981;30:515–524. PubMed

Vyskočil F, Teisinger J, Dlouhá H. Importance of cardiac cell membranes in vanadate-induced NADH oxidation - Reply. Nature. 1981;294:288. doi: 10.1038/294288b0. PubMed DOI

Vyskočil F, Teisinger J, Dlouhá H. The disparity between effects of vanadate (V) and vanadyl (IV) ions on (Na+-K+)-ATPase and K+-phosphatase in skeletal muscle. Biochem Biophys Res Commun. 1981;100:982–987. doi: 10.1016/0006-291X(81)91920-3. PubMed DOI

Teisinger J, Vyskočil F, Dlouhá H. The activation of membrane ATPase by ouabain in several tissues of the golden hamster (Mesocricetus auratus) in the absence of potassium. Experientia. 1981;37:383–384. doi: 10.1007/BF01959875. PubMed DOI

Vyskočil F, Donselaar Y, Teisinger J, Dlouhá H. Temperature dependence of membrane Na+-K+-ATPase and effect of ouabain in golden hamster and mouse diaphragm. Cryobiology. 1981;18:100–101. doi: 10.1016/0011-2240(81)90057-2. DOI

Dlouhá H, Donselaar Y, Teisinger J, Vyskočil F. Effect of temperature and ouabain on the Na+-K+ activated membrane ATPase and electrogenic ionic pump of the golden hamster and mouse diaphragm. Physiol Bohemoslov. 1980;29:543–552. PubMed

Vyklický L, Vyskočil F, Kolaj M, Jastreboff P. Primary afferent depolarization and changes in extracellular potassium concentration induced by L-glutamate and L-proline in the isolated spinal cord of the frog. Neurosci Lett. 1982;32:159–164. doi: 10.1016/0304-3940(82)90267-1. PubMed DOI

Shabunova I, Vyskočil F. Postdenervation changes of intracellular potassium and sodium measured by ion selective microelectrodes in rat soleus and extensor digitorum longus muscle fibres. Pflugers Arch. 1982;394:161–164. doi: 10.1007/BF00582919. PubMed DOI

Zemková H, Teisinger J, Vyskočil F. The comparison of vanadyl (IV) and insulin-induced hyperpolarization of the mammalian muscle cell. Biochim Biophys Acta. 1982;720:405–410. doi: 10.1016/0167-4889(82)90119-7. PubMed DOI

Vyskočil F, Pilař J, Zemková H, Teisinger J. Reduction of vanadate to vanadyl by methylene-blue, imipramine, and chlorpromazine in absence of NADH. Lancet. 1982;1:1078–1079. doi: 10.1016/S0140-6736(82)92141-9. PubMed DOI

Vyskočil F, Pilař J, Zemková H, Svoboda P, Vítek V, Teisinger J. Bleomycin stimulates both membrane (Na+-K+) ATPase and electrogenic (Na+-K+) pump and partially removes the inhibition by vanadium ions. Biochem Biophys Res Commun. 1983;116:783–790. doi: 10.1016/0006-291X(83)90593-4. PubMed DOI

Svoboda P, Teisinger J, Pilař J, Vyskočil F. Vanadyl (VO2+) and vanadate (VO3−) ions inhibit the brain microsomal Na,K-ATPase with similar affinities. Protection by transferrin and noradrenaline. Biochem Pharmacol. 1984;33:2485–2891. doi: 10.1016/0006-2952(84)90722-6. PubMed DOI

Svoboda P, Teisinger J, Vyskočil F. Vanadyl (VO2+) induced lipoperoxidation in the brain microsomal fraction is not related to VO2+ inhibition of Na,K-ATPase. Biochem Pharmacol. 1984;33:2493–2497. doi: 10.1016/0006-2952(84)90723-8. PubMed DOI

Edwards C, Vyskočil F. The effects of the replacement of K+ by Tl+, Rb+, and NH4+ on the muscle membrane potential. Gen Physiol Biophys. 1984;3:259–264. PubMed

Zemková H, Teisinger J, Vyskočil F. Hyperpolarization of mouse skeletal muscle plasma membrane induced by extracellular NADH. Biochim Biophys Acta. 1984;775:64–70. doi: 10.1016/0005-2736(84)90235-9. PubMed DOI

Teisinger J, Zemková H, Vyskočil F. The effect of anion channel blockers on enzymatic activity of Na+/K+-ATPase and the electrogenic Na+/K+ pump. FEBS Lett. 1984;175:275–278. doi: 10.1016/0014-5793(84)80750-4. PubMed DOI

Teisinger J, Zemková H, Vyskočil F. The effect of anion transport inhibitors on enzymatic Na+/K+-ATPase and electrogenic Na+/K+ pump aktivity. Physiol Bohemoslov. 1985;34:282.

Zemková H, Svoboda P, Teisinger J, Vyskočil F. On the mechanism of catecholamine-induced hyperpolarization of skeletal muscle cells. Naunyn Schmiedebergs Arch Pharmacol. 1985;329:18–23. doi: 10.1007/BF00695186. PubMed DOI

Vyskočil F, Vyklický L, Jirmanová I, Ujec E. Neuromuscular junction in the larva of the fly (Sarcophaga bullata) Mol Physiol. 1985;7:127–142.

Vyklický L, Jr, Krůšek J, Vyklický L, Vyskočil F. Spider venom of Araneus opens and desensitizes glutamate channels in chick spinal cord neurones. Neurosci Lett. 1986;68:227–231. doi: 10.1016/0304-3940(86)90147-3. PubMed DOI

Slavíček J, Vyskočil F. Extracellular K+ and Ca2+ activities measured during the action of negative pressure under a suction electrode placed on the surface of frog heart ventricle. Physiol Bohemoslov. 1985;34:201–207. PubMed

Giniatullin RA, Khamitov G, Khazipov R, Magazanik LG, Nikolsky EE, Snetkov VA, Vyskočil F. Development of desensitization during repetitive end-plate activity and single end-plate currents in frog muscle. J Physiol. 1989;412:113–122. doi: 10.1113/jphysiol.1989.sp017606. PubMed DOI PMC

Zemková H, Teisinger J, Almon RR, Vejsada R, Hník P, Vyskočil F. Immobilization atrophy and membrane properties in rat skeletal muscle fibres. Pflugers Arch. 1990;416:126–129. doi: 10.1007/BF00370233. PubMed DOI

Nikolsky EE, Bukharaeva EA, Strunsky EG, Vyskočil F. Depression of miniature endplate potential frequency by acetylcholine and its analogues in frog. Br J Pharmacol. 1991;104:1024–1032. doi: 10.1111/j.1476-5381.1991.tb12544.x. PubMed DOI PMC

Kosař E, Teisinger J, Vyskočil F, Vanĕček J. Chemical modifications of melatonin receptors in chicken brain. J Neurochem. 1994;63:662–670. doi: 10.1046/j.1471-4159.1994.63020662.x. PubMed DOI

Teisinger J, Zemková H, Svoboda P, Amler E, Vyskočil F. Ouabain binding, ATP hydrolysis, and Na+,K+-pump activity during chemical modification of brain and muscle Na+,K+-ATPase. J Neurochem. 1992;58:1066–1072. doi: 10.1111/j.1471-4159.1992.tb09363.x. PubMed DOI