The effect of vanadate on the electrogenic Na+/K+ pump, intracellular Na+ concentration and electrophysiological characteristics of mouse skeletal muscle fibre
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
PubMed
6261275
Knihovny.cz E-zdroje
- MeSH
- akční potenciály účinky léků MeSH
- bránice metabolismus MeSH
- draslík metabolismus MeSH
- elektrofyziologie MeSH
- membránové potenciály účinky léků MeSH
- myši MeSH
- sodík metabolismus MeSH
- sodíko-draslíková ATPasa metabolismus MeSH
- svaly enzymologie metabolismus fyziologie MeSH
- techniky in vitro MeSH
- vanad farmakologie MeSH
- vanadáty MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- draslík MeSH
- sodík MeSH
- sodíko-draslíková ATPasa MeSH
- vanad MeSH
- vanadáty MeSH
The present study reports a discrepancy between the effects of vanadate on the membrane Na+-K+-ATPase and the Na+/K+ pump of the skeletal muscle. Vanadate in concentration 4 X 10(-6) mol/l which is necessary to block the enzyme Na+-K+-ATPase activity of membrane fractions failed to inhibit the electrogenic Na+/K+ pump of intact muscle cells. The effect of vanadate on the electrophysiological parameters of the muscle fibre membrane required much higher vanadate levels, but again, Na+/K+ pump was still active. Vanadate in concentrations 4 X 10(-4) and 4 X 10(-5) mol/l depolarized the membrane potential and decreased the membrane resistance [apparently in consequence of enhanced passive membrane permeability for Na+ ions]. Action potentials and the electrical excitability of the muscle fibre membrane were reduced by these vanadate concentrations.
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