The effect of vanadate on the electrogenic Na+/K+ pump, intracellular Na+ concentration and electrophysiological characteristics of mouse skeletal muscle fibre
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
6261275
Knihovny.cz E-resources
- MeSH
- Action Potentials drug effects MeSH
- Diaphragm metabolism MeSH
- Potassium metabolism MeSH
- Electrophysiology MeSH
- Membrane Potentials drug effects MeSH
- Mice MeSH
- Sodium metabolism MeSH
- Sodium-Potassium-Exchanging ATPase metabolism MeSH
- Muscles enzymology metabolism physiology MeSH
- In Vitro Techniques MeSH
- Vanadium pharmacology MeSH
- Vanadates MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Potassium MeSH
- Sodium MeSH
- Sodium-Potassium-Exchanging ATPase MeSH
- Vanadium MeSH
- Vanadates MeSH
The present study reports a discrepancy between the effects of vanadate on the membrane Na+-K+-ATPase and the Na+/K+ pump of the skeletal muscle. Vanadate in concentration 4 X 10(-6) mol/l which is necessary to block the enzyme Na+-K+-ATPase activity of membrane fractions failed to inhibit the electrogenic Na+/K+ pump of intact muscle cells. The effect of vanadate on the electrophysiological parameters of the muscle fibre membrane required much higher vanadate levels, but again, Na+/K+ pump was still active. Vanadate in concentrations 4 X 10(-4) and 4 X 10(-5) mol/l depolarized the membrane potential and decreased the membrane resistance [apparently in consequence of enhanced passive membrane permeability for Na+ ions]. Action potentials and the electrical excitability of the muscle fibre membrane were reduced by these vanadate concentrations.
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