Voltage-dependent P/Q-type calcium channels at the frog neuromuscular junction
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21812515
DOI
10.33549/physiolres.932219
PII: 932219
Knihovny.cz E-resources
- MeSH
- Ion Channel Gating physiology MeSH
- Membrane Potentials physiology MeSH
- Neuromuscular Junction physiology MeSH
- Synaptic Transmission physiology MeSH
- Rana ridibunda MeSH
- Calcium metabolism MeSH
- Calcium Signaling physiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Calcium MeSH
It is well known that antagonists of N-type voltage-gated calcium channels inhibit the evoked quantal release of acetylcholine in amphibian neuromuscular synapses. This, however, does not exclude the functional expression of other types of voltage-gated calcium channels in these nerve terminals. Using immunocytochemistry, we detected the expression of the alpha1A subunit of P/Q-type calcium channels (that is otherwise typical of mammalian motor nerve endings) in the frog neuromuscular junction. In addition, we demonstrated that the P/Q-type channel blocker omega-agatoxin IVA (20 nM) reduced the action potential-induced calcium transient and significantly decreased both spontaneous and evoked mediator release. Our data indicates the functional expression of P/Q-type calcium channels in the frog motor nerve ending which participate in acetylcholine release.
References provided by Crossref.org
From Frog Muscle to Brain Neurons: Joys and Sorrows in Neuroscience
