Spontaneous quantal and non-quantal release of acetylcholine at mouse endplate during onset of hypoxia
Jazyk angličtina Země Česko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
15826238
Knihovny.cz E-zdroje
- MeSH
- acetylcholin metabolismus MeSH
- akční potenciály fyziologie MeSH
- hypoxie buňky fyziologie MeSH
- kosterní svalová vlákna metabolismus fyziologie MeSH
- membránové potenciály fyziologie MeSH
- myši MeSH
- nervosvalová ploténka metabolismus fyziologie MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- acetylcholin MeSH
At 20 (0)C, both quantal and non-quantal spontaneous acetylcholine release (expressed as miniature endplate potential frequency [f-MEPPs] and the H-effect, respectively) increased during the first 30 min of hypoxia in solution with normal extracellular calcium ([Ca(2+)](o) = 2.0 mM). The hypoxia-induced tenfold increase of the f-MEPPs was virtually absent in low calcium solution([Ca(2+)](o) = 0.4 mM) whereas there was still a significant increment of non-quantal release. This indicates that each of these two processes of acetylcholine release is influenced by mechanisms with different oxygen sensitivity. The rise of f-MEPPs during the onset of hypoxia apparently requires Ca(2+) entry into the nerve terminal, whereas the non-quantal release can be increased by another factors such as a lower level of ATP.
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